Detailed information of TA system TA112888

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 694656..695491
• Replicon: chromosome
• Accession: NZ_CP032989
• Organism: Escherichia coli strain W2-5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A763DSF8
• Locus tag: D9V55_RS03510
• Protein ID: WP_047928871.1
• Coordinates: 695114..695491 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: D9V55_RS03505
• Protein ID: WP_047928872.1
• Coordinates: 694656..695024 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V55_RS03475	691538..692356	+	819	WP_072658182.1	DUF945 domain-containing protein	-
D9V55_RS03485	692694..693167	+	474	WP_001355870.1	antirestriction protein	-
D9V55_RS03490	693183..693659	+	477	WP_001186771.1	RadC family protein	-
D9V55_RS03495	693722..693943	+	222	WP_000692327.1	DUF987 domain-containing protein	-
D9V55_RS03500	693962..694606	+	645	WP_047928873.1	hypothetical protein	-
D9V55_RS03505	694656..695024	+	369	WP_047928872.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D9V55_RS03510	695114..695491	+	378	WP_047928871.1	TA system toxin CbtA family protein	Toxin
D9V55_RS03515	695488..695637	+	150	Protein_665	hypothetical protein	-
D9V55_RS03520	695713..695910	+	198	WP_096093965.1	DUF957 domain-containing protein	-
D9V55_RS03525	695995..696840	+	846	WP_072650597.1	DUF4942 domain-containing protein	-
D9V55_RS03535	697140..697646	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
D9V55_RS03540	697724..699565	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13941.88 Da
• Isoelectric Point: 7.3222

>T112888 WP_047928871.1 NZ_CP032989:695114-695491 [Escherichia coli]
MKTLPVLPGQAAGSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNGITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T112888 NZ_CP032989:695114-695491 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGGCATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13757.47 Da
• Isoelectric Point: 5.0907

>AT112888 WP_047928872.1 NZ_CP032989:694656-695024 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEDNQLHYLADRAGIRGRFSDADAYHLDQALPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT112888 NZ_CP032989:694656-695024 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGACAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAAGCCCTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A763DSF8