Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 49283..49805 Replicon plasmid p2_BE2-5
Accession NZ_CP032988
Organism Escherichia coli strain BE2-5

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag D9V56_RS23530 Protein ID WP_000220560.1
Coordinates 49283..49564 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag D9V56_RS23535 Protein ID WP_000121743.1
Coordinates 49554..49805 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V56_RS23500 45693..47129 - 1437 WP_000105383.1 glutathione synthase -
D9V56_RS23510 47396..48100 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
D9V56_RS23520 48193..48501 - 309 Protein_51 RepB family plasmid replication initiator protein -
D9V56_RS23525 48511..49208 - 698 Protein_52 IS1 family transposase -
D9V56_RS23530 49283..49564 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D9V56_RS23535 49554..49805 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
D9V56_RS23550 51039..51875 + 837 WP_001050931.1 replication initiation protein -
D9V56_RS23555 51915..52361 + 447 Protein_56 hypothetical protein -
D9V56_RS23560 52429..52854 + 426 WP_000202942.1 hypothetical protein -
D9V56_RS23565 52851..53828 - 978 WP_024245156.1 hypothetical protein -
D9V56_RS23570 53853..54134 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / qnrS2 - 1..84688 84688


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T112885 WP_000220560.1 NZ_CP032988:c49564-49283 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T112885 NZ_CP032988:c49564-49283 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT112885 WP_000121743.1 NZ_CP032988:c49805-49554 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT112885 NZ_CP032988:c49805-49554 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References