Detailed information of TA system TA112883

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3678682..3679469
• Replicon: chromosome
• Accession: NZ_CP032986
• Organism: Escherichia coli strain BE2-5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A377E3D6
• Locus tag: D9V56_RS18195
• Protein ID: WP_001194695.1
• Coordinates: 3679092..3679469 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: D9V56_RS18190
• Protein ID: WP_072644718.1
• Coordinates: 3678682..3679041 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V56_RS18150	3673786..3674991	-	1206	Protein_3443	IS66 family transposase	-
D9V56_RS18155	3674988..3675215	-	228	Protein_3444	IS66 family insertion sequence element accessory protein TnpB	-
D9V56_RS18160	3675212..3675595	-	384	WP_044067157.1	transposase	-
D9V56_RS18165	3675875..3676750	+	876	WP_053266523.1	GTPase family protein	-
D9V56_RS24105	3677011..3677925	+	915	WP_159189431.1	antirestriction protein	-
D9V56_RS18180	3677937..3678416	+	480	WP_021553292.1	DNA repair protein RadC	-
D9V56_RS18185	3678437..3678658	+	222	WP_044068431.1	DUF987 domain-containing protein	-
D9V56_RS18190	3678682..3679041	+	360	WP_072644718.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D9V56_RS18195	3679092..3679469	+	378	WP_001194695.1	TA system toxin CbtA family protein	Toxin
D9V56_RS18200	3679466..3679957	+	492	WP_000777682.1	hypothetical protein	-
D9V56_RS18205	3679997..3680191	+	195	WP_032155094.1	DUF957 domain-containing protein	-
D9V56_RS18210	3680272..3681116	+	845	Protein_3454	DUF4942 domain-containing protein	-
D9V56_RS18220	3681459..3682190	-	732	WP_001297205.1	DNA polymerase III subunit epsilon	-
D9V56_RS18225	3682255..3682722	+	468	WP_000917883.1	ribonuclease HI	-
D9V56_RS18230	3682719..3683441	-	723	WP_001297210.1	class I SAM-dependent methyltransferase	-
D9V56_RS18235	3683475..3684230	+	756	WP_077492736.1	hydroxyacylglutathione hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	tssA / hcp1/tssD1	3649975..3684230	34255
	-	flank	IS/Tn	-	-	3673786..3674847	1061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14180.97 Da
• Isoelectric Point: 7.4209

>T112883 WP_001194695.1 NZ_CP032986:3679092-3679469 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Nucleotide

• Length: 378 bp

>T112883 NZ_CP032986:3679092-3679469 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13125.04 Da
• Isoelectric Point: 7.0261

>AT112883 WP_072644718.1 NZ_CP032986:3678682-3679041 [Escherichia coli]
MSNKTPIVNHDITEPWWGLKRSITPCFGARLVQAGNCLHYLADRASITGQFSDADLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 360 bp

>AT112883 NZ_CP032986:3678682-3679041 [Escherichia coli]
TTGTCGAATAAAACACCGATAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGGCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
CGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Toxin

• AlphaFold DB: A0A377E3D6