Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2389163..2389801 Replicon chromosome
Accession NZ_CP032986
Organism Escherichia coli strain BE2-5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag D9V56_RS11850 Protein ID WP_000813794.1
Coordinates 2389625..2389801 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag D9V56_RS11845 Protein ID WP_001270286.1
Coordinates 2389163..2389579 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V56_RS11825 2384315..2385256 - 942 WP_001251315.1 ABC transporter permease -
D9V56_RS11830 2385257..2386270 - 1014 WP_077491391.1 ABC transporter ATP-binding protein -
D9V56_RS11835 2386288..2387433 - 1146 WP_077491393.1 ABC transporter substrate-binding protein -
D9V56_RS11840 2387678..2389084 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
D9V56_RS11845 2389163..2389579 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
D9V56_RS11850 2389625..2389801 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
D9V56_RS11855 2390023..2390253 + 231 WP_000494244.1 YncJ family protein -
D9V56_RS11860 2390345..2392306 - 1962 WP_077491395.1 23S rRNA 5-hydroxycytidine C2501 synthase -
D9V56_RS11865 2392379..2392915 - 537 WP_000429150.1 DNA-binding transcriptional regulator SutR -
D9V56_RS11870 2392968..2394182 + 1215 WP_072169390.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T112878 WP_000813794.1 NZ_CP032986:c2389801-2389625 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T112878 NZ_CP032986:c2389801-2389625 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT112878 WP_001270286.1 NZ_CP032986:c2389579-2389163 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT112878 NZ_CP032986:c2389579-2389163 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References