Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1029770..1030353 Replicon chromosome
Accession NZ_CP032986
Organism Escherichia coli strain BE2-5

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag D9V56_RS05090 Protein ID WP_000254738.1
Coordinates 1030018..1030353 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag D9V56_RS05085 Protein ID WP_000581941.1
Coordinates 1029770..1030018 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V56_RS05075 1026109..1027410 + 1302 WP_077492052.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
D9V56_RS05080 1027458..1029692 + 2235 WP_000226815.1 GTP pyrophosphokinase -
D9V56_RS05085 1029770..1030018 + 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
D9V56_RS05090 1030018..1030353 + 336 WP_000254738.1 endoribonuclease MazF Toxin
D9V56_RS05095 1030424..1031215 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
D9V56_RS05100 1031443..1033080 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
D9V56_RS05105 1033168..1034466 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T112868 WP_000254738.1 NZ_CP032986:1030018-1030353 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T112868 NZ_CP032986:1030018-1030353 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT112868 WP_000581941.1 NZ_CP032986:1029770-1030018 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT112868 NZ_CP032986:1029770-1030018 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References