Detailed information of TA system TA112856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 88505..89120
• Replicon: plasmid pAt1D1108a
• Accession: NZ_CP032923
• Organism: Agrobacterium tumefaciens strain 1D1108

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: At1D1108_RS23995
• Protein ID: WP_080803121.1
• Coordinates: 88824..89120 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1S7NDM2
• Locus tag: At1D1108_RS23990
• Protein ID: WP_065689125.1
• Coordinates: 88505..88819 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
At1D1108_RS23970	84497..85906	-	1410	WP_080802435.1	amidase	-
At1D1108_RS23975	86016..86582	+	567	WP_080802441.1	TetR/AcrR family transcriptional regulator	-
At1D1108_RS23980	86788..87099	+	312	WP_080802444.1	helix-turn-helix domain-containing protein	-
At1D1108_RS23985	87092..88345	+	1254	WP_080803118.1	type II toxin-antitoxin system HipA family toxin	-
At1D1108_RS23990	88505..88819	-	315	WP_065689125.1	putative addiction module antidote protein	Antitoxin
At1D1108_RS23995	88824..89120	-	297	WP_080803121.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
At1D1108_RS24000	89646..90855	+	1210	Protein_97	Fic family protein	-
At1D1108_RS24005	91051..91305	+	255	WP_080802451.1	type II toxin-antitoxin system ParD family antitoxin	-
At1D1108_RS24010	91305..91598	+	294	WP_080802455.1	type II toxin-antitoxin system RelE/ParE family toxin	-
At1D1108_RS24015	91736..92668	-	933	WP_080802458.1	hypothetical protein	-
At1D1108_RS24020	92575..93831	-	1257	WP_080802461.1	MSMEG_0569 family flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..502274	502274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 14588.50 Da
• Isoelectric Point: 5.5500

>T112856 WP_080803121.1 NZ_CP032923:c89120-88824 [Agrobacterium tumefaciens]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 297 bp

>T112856 NZ_CP032923:c89120-88824 [Agrobacterium tumefaciens]
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Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 15471.56 Da
• Isoelectric Point: 5.5500

>AT112856 WP_065689125.1 NZ_CP032923:c88819-88505 [Agrobacterium tumefaciens]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 315 bp

>AT112856 NZ_CP032923:c88819-88505 [Agrobacterium tumefaciens]
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Structures

Antitoxin

• AlphaFold DB: A0A1S7NDM2