Detailed information of TA system TA112844

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2504193..2504850
• Replicon: chromosome
• Accession: NZ_CP032916
• Organism: Enterobacter roggenkampii strain ECY546

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W7P4L3
• Locus tag: D8675_RS13180
• Protein ID: WP_023326024.1
• Coordinates: 2504440..2504850 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W7NX65
• Locus tag: D8675_RS13175
• Protein ID: WP_006178375.1
• Coordinates: 2504193..2504459 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8675_RS13155	2499627..2501060	-	1434	WP_045371270.1	6-phospho-beta-glucosidase	-
D8675_RS13160	2501177..2501908	-	732	WP_008499714.1	MurR/RpiR family transcriptional regulator	-
D8675_RS13165	2502174..2502833	+	660	WP_021242052.1	hemolysin III family protein	-
D8675_RS13170	2502918..2503898	-	981	WP_008499712.1	tRNA-modifying protein YgfZ	-
D8675_RS13175	2504193..2504459	+	267	WP_006178375.1	FAD assembly factor SdhE	Antitoxin
D8675_RS13180	2504440..2504850	+	411	WP_023326024.1	protein YgfX	Toxin
D8675_RS13185	2504857..2505378	-	522	WP_008499710.1	flavodoxin FldB	-
D8675_RS13190	2505481..2506377	+	897	WP_008499709.1	site-specific tyrosine recombinase XerD	-
D8675_RS13195	2506406..2507119	+	714	WP_008499708.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
D8675_RS13200	2507125..2508858	+	1734	WP_023326023.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16063.06 Da
• Isoelectric Point: 10.9468

>T112844 WP_023326024.1 NZ_CP032916:2504440-2504850 [Enterobacter roggenkampii]
VVLWQSDLRVSWRSQWMSLLLHGLVAAIVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNCGMMLRLRKVDGGRCQHLWLAADSMDAAEWRDLRRMLLQQTTQG

Nucleotide

• Length: 411 bp

>T112844 NZ_CP032916:2504440-2504850 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CATTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGCTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGCTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAGCAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10569.05 Da
• Isoelectric Point: 5.1340

>AT112844 WP_006178375.1 NZ_CP032916:2504193-2504459 [Enterobacter roggenkampii]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT112844 NZ_CP032916:2504193-2504459 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: W7P4L3

Antitoxin

• AlphaFold DB: W7NX65