112839

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 177150..177672 Replicon plasmid pY546
Accession NZ_CP032915
Organism Enterobacter roggenkampii strain ECY546

Toxin (Protein)


Gene name stbE Uniprot ID A0A4R0GPV2
Locus tag D8675_RS00985 Protein ID WP_032731992.1
Coordinates 177388..177672 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4ZSR4
Locus tag D8675_RS00980 Protein ID WP_004187025.1
Coordinates 177150..177398 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8675_RS00960 172183..172335 + 153 Protein_179 IS3 family transposase -
D8675_RS00965 172568..173701 - 1134 WP_022651297.1 glutathione-independent formaldehyde dehydrogenase -
D8675_RS00970 174160..175758 - 1599 WP_050597293.1 sensor domain-containing diguanylate cyclase -
D8675_RS00975 175982..176905 + 924 WP_032731993.1 IS5 family transposase -
D8675_RS00980 177150..177398 + 249 WP_004187025.1 plasmid stabilization protein Antitoxin
D8675_RS00985 177388..177672 + 285 WP_032731992.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8675_RS00990 177907..178565 + 659 Protein_185 IS1 family transposase -
D8675_RS00995 178614..179597 - 984 Protein_186 IS630 family transposase -
D8675_RS01000 179952..180329 + 378 Protein_187 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid formA - 1..208740 208740
- inside IScluster/Tn formA - 169455..189013 19558


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11017.75 Da        Isoelectric Point: 10.5388

>T112839 WP_032731992.1 NZ_CP032915:177388-177672 [Enterobacter roggenkampii]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGIGK
RENDDIYNATLNRN

Download         Length: 285 bp

>T112839 NZ_CP032915:177388-177672 [Enterobacter roggenkampii]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCGCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAGCTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATACCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCATAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9011.24 Da        Isoelectric Point: 3.9947

>AT112839 WP_004187025.1 NZ_CP032915:177150-177398 [Enterobacter roggenkampii]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Download         Length: 249 bp

>AT112839 NZ_CP032915:177150-177398 [Enterobacter roggenkampii]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0GPV2

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZSR4

References