Detailed information of TA system TA112797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 2527531..2528444
• Replicon: chromosome
• Accession: NZ_CP032897
• Organism: Enterobacter kobei strain WCHEK045523

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: D9T11_RS13630
• Protein ID: WP_121527683.1
• Coordinates: 2527531..2528004 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J0PMR8
• Locus tag: D9T11_RS13635
• Protein ID: WP_023337017.1
• Coordinates: 2528001..2528444 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9T11_RS13610	2523699..2525000	+	1302	WP_121527679.1	type I-F CRISPR-associated protein Csy1	-
D9T11_RS13615	2524997..2525932	+	936	WP_121527680.1	type I-F CRISPR-associated protein Csy2	-
D9T11_RS13620	2525944..2526945	+	1002	WP_121527681.1	type I-F CRISPR-associated protein Csy3	-
D9T11_RS13625	2526955..2527509	+	555	WP_121527682.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
D9T11_RS13630	2527531..2528004	-	474	WP_121527683.1	RES family NAD+ phosphorylase	Toxin
D9T11_RS13635	2528001..2528444	-	444	WP_023337017.1	DUF2384 domain-containing protein	Antitoxin
D9T11_RS13640	2530442..2530660	-	219	WP_002211347.1	translation initiation factor IF-1	-
D9T11_RS13645	2530945..2531649	-	705	WP_121527684.1	leucyl/phenylalanyl-tRNA--protein transferase	-
D9T11_RS13650	2531695..2533416	-	1722	WP_023332533.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (15-147)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17584.89 Da
• Isoelectric Point: 5.4985

>T112797 WP_121527683.1 NZ_CP032897:c2528004-2527531 [Enterobacter kobei]
MIFYRLVTGRYASEAWSGSGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVRKDTVLNQYQLFSIDIPDDQIDYLDKA
WLPEDWQENPAPVSTMDLGTGWLQAKSALALILPSCIIPYENNAILNPLHPSFHTALNSVQQLPFNFDTRLAEKTAP

Nucleotide

• Length: 474 bp

>T112797 NZ_CP032897:c2528004-2527531 [Enterobacter kobei]
ATGATTTTTTATCGACTGGTAACGGGGCGCTATGCCAGCGAAGCGTGGAGCGGAAGTGGTGCAAACCAGTATGGCGGGCG
GTGGAATCATAAAGGCCATCCAGCTGTGTATGTGTCCACCTCCATTTCACTGGCCTCACTGGAGATTTTGGTGCACGTTC
GAAAAGATACTGTACTGAATCAGTACCAGCTTTTCAGCATTGATATCCCGGATGACCAGATTGATTACCTGGATAAAGCG
TGGCTCCCCGAGGACTGGCAGGAAAATCCGGCCCCCGTATCAACGATGGATCTGGGAACCGGTTGGTTGCAGGCTAAAAG
CGCCCTCGCGCTCATCTTACCCTCCTGCATCATTCCTTATGAAAACAATGCCATCCTTAACCCCTTACATCCGTCCTTCC
ACACGGCGCTGAATTCTGTTCAGCAACTGCCCTTTAATTTTGATACCCGCCTGGCTGAAAAAACCGCACCCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16349.68 Da
• Isoelectric Point: 7.2062

>AT112797 WP_023337017.1 NZ_CP032897:c2528444-2528001 [Enterobacter kobei]
MRTWIPAQKPADNALWRYAGLPANRGMRLIEFLNQGLPVSVLDNIHEWTAMSKADILRVTGINERNVARRKSAGRTLTPD
ESERVARFVRVLDAAVDYFGSKEEAWNWLQAPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT112797 NZ_CP032897:c2528444-2528001 [Enterobacter kobei]
ATGAGAACATGGATCCCCGCGCAGAAACCTGCTGATAACGCGCTTTGGCGGTACGCCGGTTTACCTGCAAACAGAGGTAT
GCGGCTGATTGAATTTCTCAATCAGGGATTACCCGTCAGCGTCCTCGACAATATCCATGAGTGGACGGCGATGTCGAAAG
CGGACATTTTGCGCGTTACCGGAATTAATGAGCGTAACGTGGCCCGCCGCAAAAGCGCGGGACGGACGCTGACGCCGGAT
GAAAGCGAACGCGTGGCCCGTTTCGTTCGGGTGCTGGATGCTGCTGTCGACTATTTTGGCAGTAAAGAAGAGGCATGGAA
CTGGCTGCAGGCACCGGTGCGCGGGCTGGGAAATGTTGCCCCTGTCGACCTGATCGCGACCGAAACCGGTGCACTGGAAG
TCACCGACTTGATTGGTCGACTCGAACATGGTGTGTTCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PMR8