Detailed information of TA system TA112784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2586542..2587180
• Replicon: chromosome
• Accession: NZ_CP032892
• Organism: Escherichia coli strain SCEC020022

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C7V31_RS15945
• Protein ID: WP_000813794.1
• Coordinates: 2587004..2587180 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C7V31_RS15940
• Protein ID: WP_001270285.1
• Coordinates: 2586542..2586958 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V31_RS15920	2581694..2582635	-	942	WP_001251313.1	ABC transporter permease	-
C7V31_RS15925	2582636..2583649	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
C7V31_RS15930	2583667..2584812	-	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
C7V31_RS15935	2585057..2586463	-	1407	WP_000760590.1	PLP-dependent aminotransferase family protein	-
C7V31_RS15940	2586542..2586958	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C7V31_RS15945	2587004..2587180	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C7V31_RS15950	2587402..2587632	+	231	WP_000494244.1	YncJ family protein	-
C7V31_RS15955	2587724..2589685	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C7V31_RS15960	2589758..2590294	-	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
C7V31_RS15965	2590347..2591561	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2591601..2592749	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T112784 WP_000813794.1 NZ_CP032892:c2587180-2587004 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T112784 NZ_CP032892:c2587180-2587004 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT112784 WP_001270285.1 NZ_CP032892:c2586958-2586542 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT112784 NZ_CP032892:c2586958-2586542 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB