Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 951168..951822 Replicon chromosome
Accession NZ_CP032892
Organism Escherichia coli strain SCEC020022

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag C7V31_RS07775 Protein ID WP_000244772.1
Coordinates 951415..951822 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C7V31_RS07770 Protein ID WP_000354046.1
Coordinates 951168..951434 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7V31_RS07745 946337..947080 + 744 WP_000951941.1 SDR family oxidoreductase -
C7V31_RS07750 947137..948570 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
C7V31_RS07755 948615..948926 + 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
C7V31_RS07760 949090..949749 + 660 WP_000250274.1 hemolysin III family protein -
C7V31_RS07765 949945..950925 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
C7V31_RS07770 951168..951434 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C7V31_RS07775 951415..951822 + 408 WP_000244772.1 protein YgfX Toxin
C7V31_RS07780 951862..952383 - 522 WP_001055874.1 flavodoxin FldB -
C7V31_RS07785 952495..953391 + 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
C7V31_RS07790 953416..954126 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C7V31_RS07795 954132..955865 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T112771 WP_000244772.1 NZ_CP032892:951415-951822 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T112771 NZ_CP032892:951415-951822 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT112771 WP_000354046.1 NZ_CP032892:951168-951434 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT112771 NZ_CP032892:951168-951434 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References