Detailed information of TA system TA112757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 43938..44539
• Replicon: plasmid pTetA_020022
• Accession: NZ_CP032890
• Organism: Escherichia coli strain SCEC020022

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: C7V31_RS02150
• Protein ID: WP_033551393.1
• Coordinates: 43938..44318 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: C7V31_RS02155
• Protein ID: WP_001190712.1
• Coordinates: 44318..44539 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V31_RS02115	39330..39539	+	210	WP_000874156.1	hypothetical protein	-
C7V31_RS02120	39650..40501	+	852	WP_000611664.1	hypothetical protein	-
C7V31_RS02125	40534..41649	-	1116	WP_033551397.1	phage antirepressor KilAC domain-containing protein	-
C7V31_RS02130	41646..42005	-	360	Protein_49	host cell division inhibitor Icd-like protein	-
C7V31_RS02135	42227..42592	-	366	Protein_50	terminase	-
C7V31_RS02140	42683..43726	-	1044	WP_033551394.1	DUF968 domain-containing protein	-
C7V31_RS02145	43754..43933	-	180	WP_000113018.1	hypothetical protein	-
C7V31_RS02150	43938..44318	-	381	WP_033551393.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C7V31_RS02155	44318..44539	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C7V31_RS02160	44722..44898	+	177	Protein_55	type I restriction-modification system subunit M N-terminal domain-containing protein	-
C7V31_RS02165	44956..45362	+	407	Protein_56	IS1 family transposase	-
C7V31_RS02170	45396..46910	-	1515	Protein_57	Tn3-like element TnAs1 family transposase	-
C7V31_RS02175	46976..47680	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C7V31_RS27215	47686..47826	+	141	WP_001044210.1	hypothetical protein	-
C7V31_RS02185	48312..49049	+	738	WP_001366550.1	recombinase family protein	-
C7V31_RS02190	49046..49270	+	225	WP_000743213.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3	-	1..91014	91014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13559.26 Da
• Isoelectric Point: 5.6277

>T112757 WP_033551393.1 NZ_CP032890:c44318-43938 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGGISVSSVADTLRRLYGSSE

Nucleotide

• Length: 381 bp

>T112757 NZ_CP032890:c44318-43938 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACTTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGTGCTGCGACTGG
CGGGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTTCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT112757 WP_001190712.1 NZ_CP032890:c44539-44318 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT112757 NZ_CP032890:c44539-44318 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGGGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6