Detailed information of TA system TA112744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3589205..3590019
• Replicon: chromosome
• Accession: NZ_CP032879
• Organism: Escherichia coli strain WCHEC000837

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: D9N32_RS19960
• Protein ID: WP_001054376.1
• Coordinates: 3589762..3590019 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: D9N32_RS19955
• Protein ID: WP_001309181.1
• Coordinates: 3589205..3589750 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N32_RS19930	3584896..3586209	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
D9N32_RS19935	3586221..3586499	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
D9N32_RS19940	3586496..3587617	-	1122	WP_000010829.1	M42 family metallopeptidase	-
D9N32_RS27815	3587862..3587978	-	117	Protein_3358	VOC family protein	-
D9N32_RS19945	3588016..3588234	-	219	Protein_3359	hypothetical protein	-
D9N32_RS19950	3588403..3589149	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
D9N32_RS19955	3589205..3589750	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
D9N32_RS19960	3589762..3590019	-	258	WP_001054376.1	hypothetical protein	Toxin
D9N32_RS19970	3590396..3590641	-	246	Protein_3363	GNAT family N-acetyltransferase	-
D9N32_RS19980	3590757..3591997	+	1241	Protein_3364	DNA helicase	-
D9N32_RS19985	3592580..3593560	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
D9N32_RS19990	3593625..3594731	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T112744 WP_001054376.1 NZ_CP032879:c3590019-3589762 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T112744 NZ_CP032879:c3590019-3589762 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT112744 WP_001309181.1 NZ_CP032879:c3589750-3589205 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT112744 NZ_CP032879:c3589750-3589205 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8