Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1994248..1995046 Replicon chromosome
Accession NZ_CP032879
Organism Escherichia coli strain WCHEC000837

Toxin (Protein)


Gene name yeeV Uniprot ID U9XMP3
Locus tag D9N32_RS11960 Protein ID WP_000854735.1
Coordinates 1994669..1995046 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M1EW42
Locus tag D9N32_RS11955 Protein ID WP_032153712.1
Coordinates 1994248..1994622 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9N32_RS11915 1989981..1990865 + 885 WP_024166630.1 50S ribosome-binding GTPase -
D9N32_RS11920 1990984..1991661 + 678 WP_023155728.1 hypothetical protein -
D9N32_RS11925 1991691..1991900 + 210 WP_023155727.1 DUF905 family protein -
D9N32_RS11930 1991991..1992809 + 819 WP_001234693.1 DUF945 domain-containing protein -
D9N32_RS11935 1992901..1993386 + 486 WP_032153711.1 antirestriction protein -
D9N32_RS11945 1993402..1993878 + 477 WP_001186715.1 RadC family protein -
D9N32_RS11950 1993947..1994168 + 222 WP_000692345.1 DUF987 domain-containing protein -
D9N32_RS11955 1994248..1994622 + 375 WP_032153712.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
D9N32_RS11960 1994669..1995046 + 378 WP_000854735.1 TA system toxin CbtA family protein Toxin
D9N32_RS11965 1995043..1995534 + 492 WP_023155722.1 hypothetical protein -
D9N32_RS11970 1995546..1995743 + 198 WP_000839265.1 DUF957 domain-containing protein -
D9N32_RS11975 1995828..1996694 + 867 WP_032153714.1 DUF4942 domain-containing protein -
D9N32_RS11980 1996766..1997029 + 264 WP_025492088.1 type II toxin-antitoxin system ParD family antitoxin -
D9N32_RS11985 1997026..1997352 + 327 WP_000779483.1 type II toxin-antitoxin system RelE/ParE family toxin -
D9N32_RS11990 1997828..1997998 + 171 Protein_1902 IS110 family transposase -
D9N32_RS11995 1998809..1999792 + 984 WP_024181915.1 KpsF/GutQ family sugar-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 papB / papI / kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / kpsT / kpsM / gspM 1931830..2015738 83908


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14061.07 Da        Isoelectric Point: 8.2904

>T112736 WP_000854735.1 NZ_CP032879:1994669-1995046 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T112736 NZ_CP032879:1994669-1995046 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13697.51 Da        Isoelectric Point: 6.6240

>AT112736 WP_032153712.1 NZ_CP032879:1994248-1994622 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT112736 NZ_CP032879:1994248-1994622 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XMP3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1EW42

References