Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 53373..53974 Replicon plasmid p1_000837
Accession NZ_CP032875
Organism Escherichia coli strain WCHEC000837

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag D9N32_RS00400 Protein ID WP_001216041.1
Coordinates 53594..53974 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag D9N32_RS00395 Protein ID WP_001190712.1
Coordinates 53373..53594 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9N32_RS27395 48623..48799 + 177 WP_000797289.1 hypothetical protein -
D9N32_RS00340 48796..48984 + 189 WP_000797279.1 hypothetical protein -
D9N32_RS00345 48986..49195 + 210 WP_000951710.1 hypothetical protein -
D9N32_RS00350 49192..49881 + 690 WP_087503531.1 DUF551 domain-containing protein -
D9N32_RS00355 49878..50138 + 261 WP_087503530.1 eaa protein -
D9N32_RS00360 50424..50840 + 417 WP_063074823.1 hypothetical protein -
D9N32_RS00365 50853..51146 + 294 WP_087503529.1 hypothetical protein -
D9N32_RS00370 51146..51454 + 309 WP_087503528.1 hypothetical protein -
D9N32_RS00375 51451..52221 + 771 WP_087503527.1 hypothetical protein -
D9N32_RS00385 52536..52787 + 252 WP_032153798.1 DNA polymerase III subunit theta -
D9N32_RS00390 52911..53300 + 390 WP_032153799.1 DNA repair protein -
D9N32_RS00395 53373..53594 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D9N32_RS00400 53594..53974 + 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
D9N32_RS00405 54010..55104 - 1095 WP_087503526.1 hypothetical protein -
D9N32_RS00410 55094..55861 - 768 WP_087503525.1 hypothetical protein -
D9N32_RS00415 55868..56530 - 663 WP_060877112.1 DUF2829 domain-containing protein -
D9N32_RS00420 56545..57039 - 495 WP_000640903.1 dUTP diphosphatase -
D9N32_RS00425 57036..57512 - 477 WP_087503524.1 hypothetical protein -
D9N32_RS00430 57509..57853 - 345 WP_087503523.1 hypothetical protein -
D9N32_RS00435 57928..58956 - 1029 WP_087503522.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92538 92538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T112711 WP_001216041.1 NZ_CP032875:53594-53974 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T112711 NZ_CP032875:53594-53974 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT112711 WP_001190712.1 NZ_CP032875:53373-53594 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT112711 NZ_CP032875:53373-53594 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGAGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References