Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5871187..5872916 Replicon chromosome
Accession NZ_CP032871
Organism Pseudomonas syringae pv. actinidiae strain P220

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2V0QPP4
Locus tag D9N00_RS28915 Protein ID WP_003379114.1
Coordinates 5871187..5871831 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A2V0QNZ0
Locus tag D9N00_RS28920 Protein ID WP_003379115.1
Coordinates 5871828..5872916 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9N00_RS28890 5866246..5867055 + 810 WP_017683789.1 zinc-dependent peptidase -
D9N00_RS28895 5867166..5867693 + 528 WP_003379107.1 inorganic diphosphatase -
D9N00_RS28900 5868039..5868770 + 732 WP_003379109.1 helix-turn-helix transcriptional regulator -
D9N00_RS28905 5869070..5870191 - 1122 WP_003379112.1 DUF91 domain-containing protein -
D9N00_RS28910 5870609..5871190 + 582 WP_017683790.1 DUF4433 domain-containing protein -
D9N00_RS28915 5871187..5871831 + 645 WP_003379114.1 DUF4433 domain-containing protein Toxin
D9N00_RS28920 5871828..5872916 + 1089 WP_003379115.1 macro domain-containing protein Antitoxin
D9N00_RS32775 5873122..5873375 - 254 Protein_5171 glycosyl transferase family 2 -
D9N00_RS28925 5873456..5874160 - 705 WP_017683791.1 ABC transporter ATP-binding protein -
D9N00_RS28930 5874157..5874933 - 777 WP_017699458.1 ABC transporter permease -
D9N00_RS28935 5875100..5876167 - 1068 WP_032685463.1 IS630 family transposase -
D9N00_RS28940 5876438..5877280 + 843 WP_003379119.1 glutathione-dependent disulfide-bond oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5875100..5875831 731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24813.38 Da        Isoelectric Point: 6.4920

>T112707 WP_003379114.1 NZ_CP032871:5871187-5871831 [Pseudomonas syringae pv. actinidiae]
MIYSSLNPEKALIWRIVHRDNLPWILDNGLHCASSYMQAPQYVNIGNADLIDKRRNRSVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLPRIEQLGLAFVFTNAHAYPDWTDYYSDLVNLDQIDWPIIQKRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

>T112707 NZ_CP032871:5871187-5871831 [Pseudomonas syringae pv. actinidiae]
ATGATCTACAGCAGTCTGAATCCAGAAAAAGCTCTGATTTGGCGAATCGTCCACCGCGATAATCTGCCTTGGATTCTGGA
CAATGGCCTGCATTGCGCAAGCTCCTACATGCAAGCACCGCAGTACGTGAACATCGGCAACGCTGACCTGATCGACAAAC
GCCGCAATCGCAGTGTTCCAATTGCTCCAGCGGGCGTACTGTCCGACTACGTGCCCTTCTACTTCACACCTTTCTCTGTG
ATGATGAAAAATATCCATTCCGGCTGGAGCGTTCAGCAGCGTAGCAATGACGAGATAGTGATTCTGGTCTCAAGCCTGCC
TCGTATTGAGCAGCTTGGCCTGGCGTTCGTCTTTACCAACGCACACGCCTACCCTGACTGGACCGATTACTACAGCGATC
TGGTGAATCTCGACCAGATCGACTGGCCTATAATCCAGAAACGAGACTTCAAGCGTGACCCTGATGATCCGCGTAAGATG
GAGCGTTATCAGGCCGAAGCGCTCATTCACCATCACCTACCGATTACAGGACTCCTGGGCATCATGTGCTACGACGATGC
AATGAAAGAACGCATTGAGCAAGACGTTGCCGCCCGAGGTCTGACTTTGCCTGTCCACGCGCGTCCGGGATGGTATTTCC
AATGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40646.66 Da        Isoelectric Point: 6.1829

>AT112707 WP_003379115.1 NZ_CP032871:5871828-5872916 [Pseudomonas syringae pv. actinidiae]
MIRFTQGNLLEADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNHVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGIDDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLCKSGVSPTVPTVREA
LKHWDGGAGAAARKSRLFDDKAIDIALKRLTTSSLSPEMPPS

Download         Length: 1089 bp

>AT112707 NZ_CP032871:5871828-5872916 [Pseudomonas syringae pv. actinidiae]
ATGATCAGATTCACCCAAGGCAATCTGCTGGAAGCAGATACCGAAGCTCTCGTCAACACGGTGAACACTGTAGGCGTAAT
GGGAAAAGGCATTGCGCTGATGTTCAAGGAGCGCTTTACCGAAAACTATCGCCTCTATTCTGCGGCGTGCAAGGCGGGTG
AGGTAGAGACCGGAAAGGTTCACGTAACGGCAGTCAACGAGCTGGACGGCCCGCGCTGGATAGTGAATTTCCCTACCAAG
CGTCACTGGCGTTCCCCCTCGCAGATGGCATGGATCACCGAAGGCTTACGGGATCTGCGCCGTTTTTTAATCGATAACCA
CGTGAAGTCCGTAGCGGTTCCACCTCTCGGGGCGGGTAATGGCGGTTTGAAATGGGTAGAGGTCCGTGAGCAGATTGTTG
ATGTCTTGAGTGATCTGGATGTCGATGTACTTGTCTTTGAACCCACTGATCAATATCTGAATGTCGCGAAGCGTAGCGGT
GTCGAGAAACTGACGCCTGCACGCGCACTCATTGCCGAACTCGTTCGGCGTTACTGGGTTTTGGGCATGGAATGCAGCCT
GCTTGAGATTCAGAAGCTCGCTTGGTTTCTGGAGCGTTCGATAGAGCAATTGCCAAGCGCCAAGAACCCCTTGGATCTCA
AATTTGTAGCCCACAAATATGGGCCATACGCCAATCGCCTTGAGCATCTGCTCGACAATCTCGATGGTAGCTACCTGCAT
TGCGATAAACGCATCAGCGACGCAGGTATTGACGACGTGATCTGGTTTGACGAGGGCCGTAAGGCTTTTCTGCAAACTTA
TCTGAAAACAGAGGCCAAGGAATATTCCCAGGCTCTGGAGCGCACCGCAGAGTTGATTGACGGCTTTGAGTCGCCTTTCG
GCATGGAACTGTTGGCGACGGTTGACTGGTTATTGTGTAAGTCGGGAGTGTCTCCAACGGTTCCGACAGTGCGTGAAGCA
TTGAAGCATTGGGATGGAGGCGCAGGTGCGGCAGCTCGGAAAAGCAGGTTGTTTGATGATAAAGCGATCGACATTGCTCT
AAAGCGATTGACTACCAGTAGTCTCAGCCCAGAGATGCCCCCTTCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0QNZ0

References