Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 5771613..5772286 Replicon chromosome
Accession NZ_CP032871
Organism Pseudomonas syringae pv. actinidiae strain P220

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V0QPW6
Locus tag D9N00_RS28455 Protein ID WP_017684243.1
Coordinates 5771867..5772286 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag D9N00_RS28450 Protein ID WP_003381346.1
Coordinates 5771613..5771870 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9N00_RS28430 5768439..5769401 + 963 WP_003383614.1 type III effector -
D9N00_RS28440 5770300..5770554 - 255 WP_003381350.1 hypothetical protein -
D9N00_RS28445 5770602..5771258 - 657 WP_017684244.1 hypothetical protein -
D9N00_RS28450 5771613..5771870 + 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
D9N00_RS28455 5771867..5772286 + 420 WP_017684243.1 type II toxin-antitoxin system VapC family toxin Toxin
D9N00_RS28460 5772327..5772960 + 634 Protein_5089 recombinase family protein -
D9N00_RS28465 5773235..5774170 + 936 WP_017704392.1 syringolide biosynthetic protein AvrD1 -
D9N00_RS28470 5774511..5775158 + 648 WP_017703697.1 histidine phosphatase family protein -
D9N00_RS28475 5775304..5776224 + 921 WP_020313563.1 TauD/TfdA family dioxygenase -
D9N00_RS28480 5776242..5777276 + 1035 WP_017684240.1 sulfotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15152.46 Da        Isoelectric Point: 4.7010

>T112706 WP_017684243.1 NZ_CP032871:5771867-5772286 [Pseudomonas syringae pv. actinidiae]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAIETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T112706 NZ_CP032871:5771867-5772286 [Pseudomonas syringae pv. actinidiae]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATTGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGATCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT112706 WP_003381346.1 NZ_CP032871:5771613-5771870 [Pseudomonas syringae pv. actinidiae]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT112706 NZ_CP032871:5771613-5771870 [Pseudomonas syringae pv. actinidiae]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPW6

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References