Detailed information of TA system TA112705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5291186..5291820
• Replicon: chromosome
• Accession: NZ_CP032871
• Organism: Pseudomonas syringae pv. actinidiae strain P220

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D9N00_RS26055
• Protein ID: WP_003380365.1
• Coordinates: 5291416..5291820 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q888H8
• Locus tag: D9N00_RS26050
• Protein ID: WP_003380366.1
• Coordinates: 5291186..5291416 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N00_RS26025	5286258..5287289	+	1032	WP_020312848.1	TRAP transporter substrate-binding protein	-
D9N00_RS26030	5287353..5287880	+	528	WP_087672763.1	TRAP transporter small permease	-
D9N00_RS26035	5287881..5289161	+	1281	WP_003380372.1	TRAP transporter large permease	-
D9N00_RS26040	5289245..5290215	+	971	Protein_4665	DMT family transporter	-
D9N00_RS26045	5290190..5291071	+	882	WP_003380368.1	aldose 1-epimerase	-
D9N00_RS26050	5291186..5291416	+	231	WP_003380366.1	antitoxin	Antitoxin
D9N00_RS26055	5291416..5291820	+	405	WP_003380365.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D9N00_RS26060	5292007..5292582	-	576	WP_003380364.1	hypothetical protein	-
D9N00_RS26065	5292581..5292769	+	189	WP_003380363.1	hypothetical protein	-
D9N00_RS26070	5293005..5293517	+	513	WP_003380362.1	sigma-70 family RNA polymerase sigma factor	-
D9N00_RS26075	5293517..5294179	+	663	WP_003380361.1	ChrR family anti-sigma-E factor	-
D9N00_RS26080	5294255..5294491	-	237	WP_003380360.1	hypothetical protein	-
D9N00_RS26085	5294556..5295812	-	1257	WP_003380359.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
D9N00_RS26090	5295908..5296390	-	483	WP_003380358.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-34)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14814.09 Da
• Isoelectric Point: 6.8615

>T112705 WP_003380365.1 NZ_CP032871:5291416-5291820 [Pseudomonas syringae pv. actinidiae]
MLKYMLDTNICIFTIKNKPVSVREAFNLHHGQLCISAITLMELVYGAEKSSSPERNLAVVEGFAARLELLPYDSAAAAHT
GMIRAELARAGTPIGPYDQMIAGHARSLGLVVITNNQREFQRVEGLRVEDWVSQ

Nucleotide

• Length: 405 bp

>T112705 NZ_CP032871:5291416-5291820 [Pseudomonas syringae pv. actinidiae]
ATGCTCAAATACATGCTCGACACCAATATTTGCATCTTCACTATCAAGAACAAGCCGGTGTCGGTACGTGAGGCCTTCAA
CCTTCATCACGGGCAGCTGTGTATCAGCGCGATCACGTTGATGGAGCTTGTTTACGGCGCAGAAAAGTCTTCCAGTCCCG
AAAGAAATCTTGCTGTTGTAGAAGGTTTTGCAGCGCGTCTTGAATTGCTTCCCTACGATAGCGCCGCCGCGGCTCACACC
GGCATGATCAGAGCCGAGCTGGCCAGAGCGGGTACACCGATTGGCCCGTACGATCAAATGATTGCAGGACATGCCCGCTC
TCTTGGCCTCGTGGTCATCACCAACAACCAGCGTGAATTCCAGCGCGTCGAGGGGCTGAGGGTAGAAGACTGGGTAAGCC
AATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8593.67 Da
• Isoelectric Point: 4.5557

>AT112705 WP_003380366.1 NZ_CP032871:5291186-5291416 [Pseudomonas syringae pv. actinidiae]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPVEQLRESL

Nucleotide

• Length: 231 bp

>AT112705 NZ_CP032871:5291186-5291416 [Pseudomonas syringae pv. actinidiae]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGTCAGGCTGTTCGACTCCCAAAAGCGGTGGCATTACCCGACGACGT
GAAACGGGTAGACGTCGTAGCAGTGGGCAGGACACGCATCATTTCCCCTGCTGGTGAAATGTGGAACAGTTGGTTCGATG
GTGAAAGTGTCAGTGATGACTTCATGGCAGAGCGCGAGCAGCCAGTTGAACAGCTGCGCGAATCACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8LU44