112701

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 42473..43087 Replicon plasmid pLKQG722
Accession NZ_CP032870
Organism Pseudomonas syringae pv. actinidiae strain P220

Toxin (Protein)


Gene name PfiT Uniprot ID A0A2V0R8Y7
Locus tag D9N00_RS00265 Protein ID WP_017685123.1
Coordinates 42473..42823 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID S6SUK0
Locus tag D9N00_RS00270 Protein ID WP_017685122.1
Coordinates 42839..43087 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9N00_RS00240 37589..38959 - 1371 WP_017684943.1 TrbI/VirB10 family protein -
D9N00_RS00245 38946..39755 - 810 WP_017684942.1 P-type conjugative transfer protein VirB9 -
D9N00_RS00250 39745..40050 - 306 WP_017706770.1 type IV secretion system protein -
D9N00_RS00255 40138..41717 + 1580 WP_085991147.1 IS3-like element ISPsy31 family transposase -
D9N00_RS00260 41757..42341 + 585 Protein_38 helix-turn-helix transcriptional regulator -
D9N00_RS00265 42473..42823 - 351 WP_017685123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D9N00_RS00270 42839..43087 - 249 WP_017685122.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D9N00_RS00275 43428..44390 + 963 WP_017685121.1 tyrosine-type recombinase/integrase -
D9N00_RS00285 44588..45655 - 1068 WP_074556923.1 IS630 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..77771 77771
- inside IScluster/Tn - - 40207..59560 19353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13012.89 Da        Isoelectric Point: 4.1953

>T112701 WP_017685123.1 NZ_CP032870:c42823-42473 [Pseudomonas syringae pv. actinidiae]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T112701 NZ_CP032870:c42823-42473 [Pseudomonas syringae pv. actinidiae]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9389.72 Da        Isoelectric Point: 9.5019

>AT112701 WP_017685122.1 NZ_CP032870:c43087-42839 [Pseudomonas syringae pv. actinidiae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK

Download         Length: 249 bp

>AT112701 NZ_CP032870:c43087-42839 [Pseudomonas syringae pv. actinidiae]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0R8Y7

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0R8I6

References