Detailed information of TA system TA112696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1392590..1393226
• Replicon: chromosome
• Accession: NZ_CP032865
• Organism: Bacillus subtilis subsp. subtilis strain N3-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: D9C18_RS07505
• Protein ID: WP_003156187.1
• Coordinates: 1392590..1392940 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: D9C18_RS07510
• Protein ID: WP_003225183.1
• Coordinates: 1392945..1393226 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9C18_RS07465	1387626..1388225	-	600	WP_003246608.1	phosphoserine phosphatase RsbX	-
D9C18_RS07470	1388225..1389013	-	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
D9C18_RS07475	1388979..1389461	-	483	WP_003234299.1	anti-sigma B factor RsbW	-
D9C18_RS07480	1389458..1389787	-	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
D9C18_RS07485	1389856..1390863	-	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
D9C18_RS07490	1390875..1391276	-	402	WP_014478899.1	serine/threonine-protein kinase RsbT	-
D9C18_RS07495	1391280..1391645	-	366	WP_003225190.1	RsbT antagonist protein RsbS	-
D9C18_RS07500	1391650..1392474	-	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
D9C18_RS07505	1392590..1392940	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D9C18_RS07510	1392945..1393226	-	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
D9C18_RS07515	1393342..1394511	-	1170	WP_014478898.1	alanine racemase	-
D9C18_RS07520	1394626..1395642	-	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
D9C18_RS07525	1395808..1396173	-	366	WP_014478897.1	holo-ACP synthase	-
D9C18_RS07530	1396268..1396867	+	600	WP_014478896.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T112696 WP_003156187.1 NZ_CP032865:c1392940-1392590 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T112696 NZ_CP032865:c1392940-1392590 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT112696 WP_003225183.1 NZ_CP032865:c1393226-1392945 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT112696 NZ_CP032865:c1393226-1392945 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32