Detailed information of TA system TA112692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2019809..2020445
• Replicon: chromosome
• Accession: NZ_CP032861
• Organism: Bacillus subtilis subsp. subtilis strain N1-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: D9C16_RS11135
• Protein ID: WP_003156187.1
• Coordinates: 2019809..2020159 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: D9C16_RS11140
• Protein ID: WP_003225183.1
• Coordinates: 2020164..2020445 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9C16_RS11095	2014845..2015444	-	600	WP_003246608.1	phosphoserine phosphatase RsbX	-
D9C16_RS11100	2015444..2016232	-	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
D9C16_RS11105	2016198..2016680	-	483	WP_003234299.1	anti-sigma B factor RsbW	-
D9C16_RS11110	2016677..2017006	-	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
D9C16_RS11115	2017075..2018082	-	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
D9C16_RS11120	2018094..2018495	-	402	WP_014478899.1	serine/threonine-protein kinase RsbT	-
D9C16_RS11125	2018499..2018864	-	366	WP_003225190.1	RsbT antagonist protein RsbS	-
D9C16_RS11130	2018869..2019693	-	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
D9C16_RS11135	2019809..2020159	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D9C16_RS11140	2020164..2020445	-	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
D9C16_RS11145	2020561..2021730	-	1170	WP_014478898.1	alanine racemase	-
D9C16_RS11150	2021845..2022861	-	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
D9C16_RS11155	2023027..2023392	-	366	WP_014478897.1	holo-ACP synthase	-
D9C16_RS11160	2023487..2024086	+	600	WP_014478896.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T112692 WP_003156187.1 NZ_CP032861:c2020159-2019809 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T112692 NZ_CP032861:c2020159-2019809 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT112692 WP_003225183.1 NZ_CP032861:c2020445-2020164 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT112692 NZ_CP032861:c2020445-2020164 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32