Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 2019809..2020445 | Replicon | chromosome |
| Accession | NZ_CP032861 | ||
| Organism | Bacillus subtilis subsp. subtilis strain N1-1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | D9C16_RS11135 | Protein ID | WP_003156187.1 |
| Coordinates | 2019809..2020159 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | D9C16_RS11140 | Protein ID | WP_003225183.1 |
| Coordinates | 2020164..2020445 (-) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D9C16_RS11095 | 2014845..2015444 | - | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
| D9C16_RS11100 | 2015444..2016232 | - | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| D9C16_RS11105 | 2016198..2016680 | - | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| D9C16_RS11110 | 2016677..2017006 | - | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| D9C16_RS11115 | 2017075..2018082 | - | 1008 | WP_014478900.1 | phosphoserine phosphatase RsbU | - |
| D9C16_RS11120 | 2018094..2018495 | - | 402 | WP_014478899.1 | serine/threonine-protein kinase RsbT | - |
| D9C16_RS11125 | 2018499..2018864 | - | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| D9C16_RS11130 | 2018869..2019693 | - | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| D9C16_RS11135 | 2019809..2020159 | - | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| D9C16_RS11140 | 2020164..2020445 | - | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| D9C16_RS11145 | 2020561..2021730 | - | 1170 | WP_014478898.1 | alanine racemase | - |
| D9C16_RS11150 | 2021845..2022861 | - | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| D9C16_RS11155 | 2023027..2023392 | - | 366 | WP_014478897.1 | holo-ACP synthase | - |
| D9C16_RS11160 | 2023487..2024086 | + | 600 | WP_014478896.1 | rhomboid family intramembrane serine protease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T112692 WP_003156187.1 NZ_CP032861:c2020159-2019809 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T112692 NZ_CP032861:c2020159-2019809 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT112692 WP_003225183.1 NZ_CP032861:c2020445-2020164 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT112692 NZ_CP032861:c2020445-2020164 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|