Detailed information of TA system TA112679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2072780..2073416
• Replicon: chromosome
• Accession: NZ_CP032860
• Organism: Bacillus subtilis subsp. subtilis strain SSJ-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: D9C14_RS10785
• Protein ID: WP_003156187.1
• Coordinates: 2073066..2073416 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: D9C14_RS10780
• Protein ID: WP_003225183.1
• Coordinates: 2072780..2073061 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9C14_RS10760	2069139..2069738	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
D9C14_RS10765	2069833..2070198	+	366	WP_120363269.1	holo-ACP synthase	-
D9C14_RS10770	2070364..2071380	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
D9C14_RS10775	2071495..2072664	+	1170	WP_121572536.1	alanine racemase	-
D9C14_RS10780	2072780..2073061	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
D9C14_RS10785	2073066..2073416	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D9C14_RS10790	2073532..2074356	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
D9C14_RS10795	2074361..2074726	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
D9C14_RS10800	2074730..2075131	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
D9C14_RS10805	2075143..2076150	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
D9C14_RS10810	2076212..2076541	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
D9C14_RS10815	2076538..2077020	+	483	WP_038427419.1	anti-sigma B factor RsbW	-
D9C14_RS10820	2076986..2077774	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
D9C14_RS10825	2077774..2078373	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T112679 WP_003156187.1 NZ_CP032860:2073066-2073416 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T112679 NZ_CP032860:2073066-2073416 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT112679 WP_003225183.1 NZ_CP032860:2072780-2073061 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT112679 NZ_CP032860:2072780-2073061 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32