Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4342..4978 Replicon chromosome
Accession NZ_CP032853
Organism Bacillus subtilis subsp. subtilis strain MH-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D9C10_RS00035 Protein ID WP_003156187.1
Coordinates 4628..4978 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag D9C10_RS00030 Protein ID WP_003225183.1
Coordinates 4342..4623 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9C10_RS00010 701..1300 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
D9C10_RS00015 1395..1760 + 366 WP_121548648.1 holo-ACP synthase -
D9C10_RS00020 1926..2942 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
D9C10_RS00025 3057..4226 + 1170 WP_014478898.1 alanine racemase -
D9C10_RS00030 4342..4623 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D9C10_RS00035 4628..4978 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D9C10_RS00040 5083..5907 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
D9C10_RS00045 5912..6277 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
D9C10_RS00050 6281..6682 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
D9C10_RS00055 6694..7701 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
D9C10_RS00060 7763..8092 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
D9C10_RS00065 8089..8571 + 483 WP_003234299.1 anti-sigma B factor RsbW -
D9C10_RS00070 8537..9325 + 789 Protein_13 RNA polymerase sigma factor SigB -
D9C10_RS00075 9325..9924 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T112676 WP_003156187.1 NZ_CP032853:4628-4978 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T112676 NZ_CP032853:4628-4978 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT112676 WP_003225183.1 NZ_CP032853:4342-4623 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT112676 NZ_CP032853:4342-4623 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References