Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 230046..230572 Replicon plasmid pSPRC-Echo1
Accession NZ_CP032842
Organism Enterobacter hormaechei strain C15117

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D8M16_RS24455 Protein ID WP_000323025.1
Coordinates 230046..230333 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D8M16_RS24460 Protein ID WP_000534858.1
Coordinates 230333..230572 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8M16_RS25470 225616..225792 - 177 WP_001371930.1 hypothetical protein -
D8M16_RS24425 226303..227247 + 945 WP_000778029.1 DUF5417 domain-containing protein -
D8M16_RS24430 227343..227945 + 603 WP_012695474.1 hypothetical protein -
D8M16_RS24435 228005..228355 + 351 WP_000743059.1 hypothetical protein -
D8M16_RS24440 228402..228605 + 204 WP_001015183.1 hypothetical protein -
D8M16_RS24450 229816..229974 - 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
D8M16_RS24455 230046..230333 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D8M16_RS24460 230333..230572 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D8M16_RS24470 230835..231758 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
D8M16_RS24475 231958..232530 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
D8M16_RS24480 233006..234244 - 1239 WP_000219087.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / blaSHV-12 / sul2 / sul1 / ere(A) / aac(6')-IIc / qacE / qnrA1 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / tet(D) / catA2 - 1..339920 339920


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T112673 WP_000323025.1 NZ_CP032842:c230333-230046 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T112673 NZ_CP032842:c230333-230046 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT112673 WP_000534858.1 NZ_CP032842:c230572-230333 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT112673 NZ_CP032842:c230572-230333 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References