Detailed information of TA system TA112671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4655486..4656102
• Replicon: chromosome
• Accession: NZ_CP032841
• Organism: Enterobacter hormaechei strain C15117

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D8M16_RS22745
• Protein ID: WP_077266491.1
• Coordinates: 4655486..4655857 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: D8M16_RS22750
• Protein ID: WP_015569912.1
• Coordinates: 4655860..4656102 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8M16_RS22730	4652986..4653888	+	903	WP_063134071.1	formate dehydrogenase subunit beta	-
D8M16_RS22735	4653885..4654520	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
D8M16_RS22740	4654517..4655446	+	930	WP_062933603.1	formate dehydrogenase accessory protein FdhE	-
D8M16_RS22745	4655486..4655857	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
D8M16_RS22750	4655860..4656102	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
D8M16_RS22755	4656301..4657221	+	921	WP_062933602.1	alpha/beta hydrolase	-
D8M16_RS22760	4657230..4658171	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
D8M16_RS22765	4658216..4658653	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D8M16_RS22770	4658650..4659531	-	882	WP_003861949.1	virulence factor BrkB family protein	-
D8M16_RS22775	4659525..4660124	-	600	WP_062933601.1	glucose-1-phosphatase	-
D8M16_RS22780	4660243..4661043	-	801	WP_062933600.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T112671 WP_077266491.1 NZ_CP032841:c4655857-4655486 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T112671 NZ_CP032841:c4655857-4655486 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT112671 WP_015569912.1 NZ_CP032841:c4656102-4655860 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT112671 NZ_CP032841:c4656102-4655860 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3