Detailed information of TA system TA112669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2289165..2289904
• Replicon: chromosome
• Accession: NZ_CP032841
• Organism: Enterobacter hormaechei strain C15117

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D8M16_RS11090
• Protein ID: WP_121478951.1
• Coordinates: 2289419..2289904 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: D8M16_RS11085
• Protein ID: WP_003857131.1
• Coordinates: 2289165..2289431 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8M16_RS11065	2284641..2285627	+	987	WP_121478947.1	DUF979 domain-containing protein	-
D8M16_RS11070	2285637..2286632	+	996	WP_121478948.1	DUF2891 domain-containing protein	-
D8M16_RS11075	2286654..2288042	-	1389	WP_121478949.1	MFS transporter	-
D8M16_RS11080	2288172..2289101	+	930	WP_121478950.1	LysR family transcriptional regulator	-
D8M16_RS11085	2289165..2289431	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
D8M16_RS11090	2289419..2289904	+	486	WP_121478951.1	GNAT family N-acetyltransferase	Toxin
D8M16_RS11095	2290098..2291501	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
D8M16_RS11100	2291530..2292162	-	633	WP_001567369.1	hypothetical protein	-
D8M16_RS11105	2292237..2293205	+	969	WP_022652343.1	IS5-like element IS903B family transposase	-
D8M16_RS11110	2293430..2294722	-	1293	WP_121478952.1	glycoside hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17526.21 Da
• Isoelectric Point: 9.9658

>T112669 WP_121478951.1 NZ_CP032841:2289419-2289904 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T112669 NZ_CP032841:2289419-2289904 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTTCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACATTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCACTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCAGAAAATATCGGGGTGCGGGCAATTATGGTTCATGCACTCACCG
AGGAAGCCAAAGGGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT112669 WP_003857131.1 NZ_CP032841:2289165-2289431 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT112669 NZ_CP032841:2289165-2289431 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCCGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCGGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9