Detailed information of TA system TA112661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 296768..297364
• Replicon: chromosome
• Accession: NZ_CP032841
• Organism: Enterobacter hormaechei strain C15117

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: D8M16_RS01460
• Protein ID: WP_090281146.1
• Coordinates: 297062..297364 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A837FG03
• Locus tag: D8M16_RS01455
• Protein ID: WP_022650216.1
• Coordinates: 296768..297055 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8M16_RS01450	295140..296771	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
D8M16_RS01455	296768..297055	-	288	WP_022650216.1	putative addiction module antidote protein	Antitoxin
D8M16_RS01460	297062..297364	-	303	WP_090281146.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D8M16_RS01465	297562..298434	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
D8M16_RS01470	298435..298707	-	273	WP_017382612.1	DUF3811 domain-containing protein	-
D8M16_RS01475	298758..299702	-	945	WP_121478500.1	ketopantoate/pantoate/pantothenate transporter PanS	-
D8M16_RS01480	299796..301145	-	1350	WP_045344657.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11439.25 Da
• Isoelectric Point: 10.5300

>T112661 WP_090281146.1 NZ_CP032841:c297364-297062 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGKSIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T112661 NZ_CP032841:c297364-297062 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAATCTATTTTAAGGACCAGGGCAAGAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10087.57 Da
• Isoelectric Point: 5.7807

>AT112661 WP_022650216.1 NZ_CP032841:c297055-296768 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT112661 NZ_CP032841:c297055-296768 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCCGTGTTTATGGCTGATGCATT
AGAAACGGGTGACTCAGCATACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FG03