Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 33722..34248 Replicon plasmid pINF237_04
Accession NZ_CP032837
Organism Klebsiella pneumoniae strain INF237

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D9K64_RS28465 Protein ID WP_000323025.1
Coordinates 33722..34009 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D9K64_RS28470 Protein ID WP_000534858.1
Coordinates 34009..34248 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K64_RS28430 29218..30906 + 1689 WP_115233593.1 cloacin -
D9K64_RS28435 30916..31173 + 258 WP_000523382.1 cloacin immunity family protein -
D9K64_RS28440 31258..31407 + 150 WP_002193747.1 colicin release lysis protein -
D9K64_RS28445 31801..32376 + 576 WP_000904911.1 recombinase family protein -
D9K64_RS28455 32778..33246 + 469 Protein_43 cytoplasmic protein -
D9K64_RS28460 33492..33650 - 159 WP_014386184.1 type I toxin-antitoxin system Hok family toxin -
D9K64_RS28465 33722..34009 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D9K64_RS28470 34009..34248 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D9K64_RS28475 34534..35231 + 698 WP_181577867.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - vat 1..44993 44993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T112658 WP_000323025.1 NZ_CP032837:c34009-33722 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T112658 NZ_CP032837:c34009-33722 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT112658 WP_000534858.1 NZ_CP032837:c34248-34009 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT112658 NZ_CP032837:c34248-34009 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References