Detailed information of TA system TA112657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 24883..25357
• Replicon: plasmid pINF237_04
• Accession: NZ_CP032837
• Organism: Klebsiella pneumoniae strain INF237

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: D9K64_RS28400
• Protein ID: WP_000262326.1
• Coordinates: 25091..25357 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: D9K64_RS28395
• Protein ID: WP_000879771.1
• Coordinates: 24883..25107 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K64_RS28350	20209..20416	+	208	Protein_26	helix-turn-helix domain-containing protein	-
D9K64_RS28360	21161..21858	+	698	WP_171893191.1	IS1 family transposase	-
D9K64_RS28365	21987..22166	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
D9K64_RS28370	22192..22620	-	429	WP_001140599.1	hypothetical protein	-
D9K64_RS28375	22784..23098	-	315	WP_001136729.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D9K64_RS28380	23086..23364	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	-
D9K64_RS28385	23685..23903	-	219	WP_002193751.1	TonB family protein	-
D9K64_RS28390	23900..24271	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
D9K64_RS28395	24883..25107	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
D9K64_RS28400	25091..25357	+	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9K64_RS28405	25414..25659	-	246	WP_004146442.1	hypothetical protein	-
D9K64_RS28415	26002..27222	+	1221	WP_115233592.1	ISL3 family transposase	-
D9K64_RS28425	27626..29113	+	1488	WP_162985506.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vat	1..44993	44993
	-	inside	IScluster/Tn	-	-	19484..27222	7738

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T112657 WP_000262326.1 NZ_CP032837:25091-25357 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T112657 NZ_CP032837:25091-25357 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT112657 WP_000879771.1 NZ_CP032837:24883-25107 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT112657 NZ_CP032837:24883-25107 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCATCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5