Detailed information of TA system TA112656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 22784..23364
• Replicon: plasmid pINF237_04
• Accession: NZ_CP032837
• Organism: Klebsiella pneumoniae strain INF237

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A060VNB1
• Locus tag: D9K64_RS28375
• Protein ID: WP_001136729.1
• Coordinates: 22784..23098 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: D9K64_RS28380
• Protein ID: WP_000093040.1
• Coordinates: 23086..23364 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K64_RS28340	18993..19424	-	432	WP_040175080.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
D9K64_RS28345	19484..20209	-	726	Protein_25	ISL3 family transposase	-
D9K64_RS28350	20209..20416	+	208	Protein_26	helix-turn-helix domain-containing protein	-
D9K64_RS28360	21161..21858	+	698	WP_171893191.1	IS1 family transposase	-
D9K64_RS28365	21987..22166	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
D9K64_RS28370	22192..22620	-	429	WP_001140599.1	hypothetical protein	-
D9K64_RS28375	22784..23098	-	315	WP_001136729.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9K64_RS28380	23086..23364	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
D9K64_RS28385	23685..23903	-	219	WP_002193751.1	TonB family protein	-
D9K64_RS28390	23900..24271	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
D9K64_RS28395	24883..25107	+	225	WP_000879771.1	TraY domain-containing protein	-
D9K64_RS28400	25091..25357	+	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D9K64_RS28405	25414..25659	-	246	WP_004146442.1	hypothetical protein	-
D9K64_RS28415	26002..27222	+	1221	WP_115233592.1	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vat	1..44993	44993
	-	inside	IScluster/Tn	-	-	19484..27222	7738

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.77 Da
• Isoelectric Point: 9.8619

>T112656 WP_001136729.1 NZ_CP032837:c23098-22784 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKTLPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDR
EMDRIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T112656 NZ_CP032837:c23098-22784 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGAGATTTACAACGCCTCCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGCCGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CATTTCTGCCGCTGGAATATCAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCGGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT112656 WP_000093040.1 NZ_CP032837:c23364-23086 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT112656 NZ_CP032837:c23364-23086 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AAGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCAGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: A0A060VNB1

Antitoxin

• AlphaFold DB: A0A2X1PRM1