Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 73434..74197 Replicon plasmid pINF237_03
Accession NZ_CP032836
Organism Klebsiella pneumoniae strain INF237

Toxin (Protein)


Gene name tacT Uniprot ID A0A080HRR7
Locus tag D9K64_RS28180 Protein ID WP_032250661.1
Coordinates 73715..74197 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A080HY83
Locus tag D9K64_RS28175 Protein ID WP_021532981.1
Coordinates 73434..73724 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K64_RS28155 71130..71855 + 726 WP_044243877.1 type-F conjugative transfer system pilin acetylase TraX -
D9K64_RS28160 71927..72523 + 597 WP_121097660.1 fertility inhibition protein FinO -
- 72849..72908 - 60 NuclAT_1 - -
- 72849..72908 - 60 NuclAT_1 - -
- 72849..72908 - 60 NuclAT_1 - -
- 72849..72908 - 60 NuclAT_1 - -
D9K64_RS28170 72953..73102 + 150 WP_001312851.1 type I toxin-antitoxin system Hok family toxin -
D9K64_RS28175 73434..73724 + 291 WP_021532981.1 DUF1778 domain-containing protein Antitoxin
D9K64_RS28180 73715..74197 + 483 WP_032250661.1 GNAT family N-acetyltransferase Toxin
D9K64_RS28185 74271..74687 - 417 WP_162985499.1 IS200/IS605 family transposase -
D9K64_RS28190 74710..75918 + 1209 WP_020947702.1 transposase -
D9K64_RS28195 76215..76472 + 258 WP_000083826.1 replication regulatory protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..76772 76772


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(28-149)

Antitoxin

Vibrio phage ICP1, Orf50

(15-90)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17742.48 Da        Isoelectric Point: 6.5974

>T112655 WP_032250661.1 NZ_CP032836:73715-74197 [Klebsiella pneumoniae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQILNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRIANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPDTLFYKI

Download         Length: 483 bp

>T112655 NZ_CP032836:73715-74197 [Klebsiella pneumoniae]
ATGGAAATAAATGTCACCGCGCCGGCATTGCTGACAGATGAGCATATACTTCAGCCATTTGACTGTGGGAATGAGGTGTT
AAGTAACTGGTTGCGTGGTCGGGCTATGAAAAACCAGATACTCAATGCTTCTCGCACGTTCGTCATTTGTCTGGAGGATA
CTTTACGTGTTGTGGGGTATTACTCATTAGCTACTGGTTCTGTCACACATGCCGAGCTTGGGCGCAGTTTGCGCCATAAT
ATGCCCAATCCTGTACCTGTTGTTCTTTTAGGGCGTCTGGCTGTCGATGTTTGTACTCAGGGACATGGTTTCGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGATTGCCAATCTGGCTGAACAGGTTGGCATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGACGCCAGAGTTTTTTATGAGCATTTTGGTTTTGTTCAGTCAGTTGTGGCACCTGATACCTTGTTTTATAAAATT
TGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 11054.51 Da        Isoelectric Point: 4.6820

>AT112655 WP_021532981.1 NZ_CP032836:73434-73724 [Klebsiella pneumoniae]
MSTAASVRKIPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNEGYDSFITQLEAPVQ
NAEGRERLMAVKPEWK

Download         Length: 291 bp

>AT112655 NZ_CP032836:73434-73724 [Klebsiella pneumoniae]
ATGTCCACTGCAGCAAGCGTCCGTAAAATACCGCGAGAAAATCAGATTAACATCCGGGCAACTGATGAGGAAAGAGCCGT
TATTGATTATGCTGCGAGCCTGGTCAATAAGAATCGTACCGATTTCATCATTGAACTGGCTTACCAGGAAGCTAAAAACA
TCATTCTGGATCAACGTTTATTTGTATTGGATAACGAAGGTTATGATTCGTTTATTACGCAACTGGAAGCTCCTGTACAG
AATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A080HRR7

Antitoxin

Source ID Structure
AlphaFold DB A0A080HY83

References