Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
Location | 73434..74197 | Replicon | plasmid pINF237_03 |
Accession | NZ_CP032836 | ||
Organism | Klebsiella pneumoniae strain INF237 |
Toxin (Protein)
Gene name | tacT | Uniprot ID | A0A080HRR7 |
Locus tag | D9K64_RS28180 | Protein ID | WP_032250661.1 |
Coordinates | 73715..74197 (+) | Length | 161 a.a. |
Antitoxin (Protein)
Gene name | tacA | Uniprot ID | A0A080HY83 |
Locus tag | D9K64_RS28175 | Protein ID | WP_021532981.1 |
Coordinates | 73434..73724 (+) | Length | 97 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
D9K64_RS28155 | 71130..71855 | + | 726 | WP_044243877.1 | type-F conjugative transfer system pilin acetylase TraX | - |
D9K64_RS28160 | 71927..72523 | + | 597 | WP_121097660.1 | fertility inhibition protein FinO | - |
- | 72849..72908 | - | 60 | NuclAT_1 | - | - |
- | 72849..72908 | - | 60 | NuclAT_1 | - | - |
- | 72849..72908 | - | 60 | NuclAT_1 | - | - |
- | 72849..72908 | - | 60 | NuclAT_1 | - | - |
D9K64_RS28170 | 72953..73102 | + | 150 | WP_001312851.1 | type I toxin-antitoxin system Hok family toxin | - |
D9K64_RS28175 | 73434..73724 | + | 291 | WP_021532981.1 | DUF1778 domain-containing protein | Antitoxin |
D9K64_RS28180 | 73715..74197 | + | 483 | WP_032250661.1 | GNAT family N-acetyltransferase | Toxin |
D9K64_RS28185 | 74271..74687 | - | 417 | WP_162985499.1 | IS200/IS605 family transposase | - |
D9K64_RS28190 | 74710..75918 | + | 1209 | WP_020947702.1 | transposase | - |
D9K64_RS28195 | 76215..76472 | + | 258 | WP_000083826.1 | replication regulatory protein RepA | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | - | - | 1..76772 | 76772 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17742.48 Da Isoelectric Point: 6.5974
>T112655 WP_032250661.1 NZ_CP032836:73715-74197 [Klebsiella pneumoniae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQILNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRIANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPDTLFYKI
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQILNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRIANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPDTLFYKI
Download Length: 483 bp
>T112655 NZ_CP032836:73715-74197 [Klebsiella pneumoniae]
ATGGAAATAAATGTCACCGCGCCGGCATTGCTGACAGATGAGCATATACTTCAGCCATTTGACTGTGGGAATGAGGTGTT
AAGTAACTGGTTGCGTGGTCGGGCTATGAAAAACCAGATACTCAATGCTTCTCGCACGTTCGTCATTTGTCTGGAGGATA
CTTTACGTGTTGTGGGGTATTACTCATTAGCTACTGGTTCTGTCACACATGCCGAGCTTGGGCGCAGTTTGCGCCATAAT
ATGCCCAATCCTGTACCTGTTGTTCTTTTAGGGCGTCTGGCTGTCGATGTTTGTACTCAGGGACATGGTTTCGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGATTGCCAATCTGGCTGAACAGGTTGGCATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGACGCCAGAGTTTTTTATGAGCATTTTGGTTTTGTTCAGTCAGTTGTGGCACCTGATACCTTGTTTTATAAAATT
TGA
ATGGAAATAAATGTCACCGCGCCGGCATTGCTGACAGATGAGCATATACTTCAGCCATTTGACTGTGGGAATGAGGTGTT
AAGTAACTGGTTGCGTGGTCGGGCTATGAAAAACCAGATACTCAATGCTTCTCGCACGTTCGTCATTTGTCTGGAGGATA
CTTTACGTGTTGTGGGGTATTACTCATTAGCTACTGGTTCTGTCACACATGCCGAGCTTGGGCGCAGTTTGCGCCATAAT
ATGCCCAATCCTGTACCTGTTGTTCTTTTAGGGCGTCTGGCTGTCGATGTTTGTACTCAGGGACATGGTTTCGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGATTGCCAATCTGGCTGAACAGGTTGGCATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGACGCCAGAGTTTTTTATGAGCATTTTGGTTTTGTTCAGTCAGTTGTGGCACCTGATACCTTGTTTTATAAAATT
TGA
Antitoxin
Download Length: 97 a.a. Molecular weight: 11054.51 Da Isoelectric Point: 4.6820
>AT112655 WP_021532981.1 NZ_CP032836:73434-73724 [Klebsiella pneumoniae]
MSTAASVRKIPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNEGYDSFITQLEAPVQ
NAEGRERLMAVKPEWK
MSTAASVRKIPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNEGYDSFITQLEAPVQ
NAEGRERLMAVKPEWK
Download Length: 291 bp
>AT112655 NZ_CP032836:73434-73724 [Klebsiella pneumoniae]
ATGTCCACTGCAGCAAGCGTCCGTAAAATACCGCGAGAAAATCAGATTAACATCCGGGCAACTGATGAGGAAAGAGCCGT
TATTGATTATGCTGCGAGCCTGGTCAATAAGAATCGTACCGATTTCATCATTGAACTGGCTTACCAGGAAGCTAAAAACA
TCATTCTGGATCAACGTTTATTTGTATTGGATAACGAAGGTTATGATTCGTTTATTACGCAACTGGAAGCTCCTGTACAG
AATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA
ATGTCCACTGCAGCAAGCGTCCGTAAAATACCGCGAGAAAATCAGATTAACATCCGGGCAACTGATGAGGAAAGAGCCGT
TATTGATTATGCTGCGAGCCTGGTCAATAAGAATCGTACCGATTTCATCATTGAACTGGCTTACCAGGAAGCTAAAAACA
TCATTCTGGATCAACGTTTATTTGTATTGGATAACGAAGGTTATGATTCGTTTATTACGCAACTGGAAGCTCCTGTACAG
AATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A080HRR7 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A080HY83 |