Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | mazEF/PRK09907-MazE |
Location | 36702..37279 | Replicon | plasmid pINF237_02 |
Accession | NZ_CP032835 | ||
Organism | Klebsiella pneumoniae strain INF237 |
Toxin (Protein)
Gene name | mazF | Uniprot ID | A0A7G3NNX6 |
Locus tag | D9K64_RS27280 | Protein ID | WP_059065587.1 |
Coordinates | 36702..37034 (-) | Length | 111 a.a. |
Antitoxin (Protein)
Gene name | mazE | Uniprot ID | A0A7G3NP82 |
Locus tag | D9K64_RS27285 | Protein ID | WP_032454911.1 |
Coordinates | 37034..37279 (-) | Length | 82 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
D9K64_RS27230 | 31774..31872 | - | 99 | Protein_40 | IS1 family transposase | - |
D9K64_RS27235 | 32048..33502 | + | 1455 | Protein_41 | Tn3 family transposase | - |
D9K64_RS27245 | 33801..34064 | + | 264 | WP_001089474.1 | hypothetical protein | - |
D9K64_RS27250 | 34054..34353 | + | 300 | WP_000119542.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
D9K64_RS29665 | 34630..34857 | - | 228 | Protein_44 | transposase | - |
D9K64_RS27265 | 35029..35726 | + | 698 | WP_181577885.1 | IS1 family transposase | - |
D9K64_RS27270 | 35728..36294 | + | 567 | WP_115272682.1 | hypothetical protein | - |
D9K64_RS27275 | 36321..36527 | - | 207 | WP_200528186.1 | hypothetical protein | - |
D9K64_RS27280 | 36702..37034 | - | 333 | WP_059065587.1 | endoribonuclease MazF | Toxin |
D9K64_RS27285 | 37034..37279 | - | 246 | WP_032454911.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
D9K64_RS27290 | 37468..37740 | + | 273 | WP_004146533.1 | hypothetical protein | - |
D9K64_RS27295 | 37721..38929 | + | 1209 | WP_121097629.1 | IS91 family transposase | - |
D9K64_RS27300 | 39049..39900 | + | 852 | WP_121097632.1 | 3'-5' exonuclease | - |
D9K64_RS27305 | 40017..40280 | + | 264 | Protein_53 | hypothetical protein | - |
D9K64_RS29565 | 41300..41449 | + | 150 | WP_168234218.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | pilW | 1..93621 | 93621 | |
- | inside | IScluster/Tn | - | - | 21172..52804 | 31632 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 11991.87 Da Isoelectric Point: 7.7494
>T112650 WP_059065587.1 NZ_CP032835:c37034-36702 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG
Download Length: 333 bp
>T112650 NZ_CP032835:c37034-36702 [Klebsiella pneumoniae]
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTGTGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGTGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTGTGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGTGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA
Antitoxin
Download Length: 82 a.a. Molecular weight: 8881.21 Da Isoelectric Point: 5.7996
>AT112650 WP_032454911.1 NZ_CP032835:c37279-37034 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L
Download Length: 246 bp
>AT112650 NZ_CP032835:c37279-37034 [Klebsiella pneumoniae]
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCAGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTGA
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCAGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7G3NNX6 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7G3NP82 |