Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 36702..37279 Replicon plasmid pINF237_02
Accession NZ_CP032835
Organism Klebsiella pneumoniae strain INF237

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag D9K64_RS27280 Protein ID WP_059065587.1
Coordinates 36702..37034 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag D9K64_RS27285 Protein ID WP_032454911.1
Coordinates 37034..37279 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K64_RS27230 31774..31872 - 99 Protein_40 IS1 family transposase -
D9K64_RS27235 32048..33502 + 1455 Protein_41 Tn3 family transposase -
D9K64_RS27245 33801..34064 + 264 WP_001089474.1 hypothetical protein -
D9K64_RS27250 34054..34353 + 300 WP_000119542.1 type II toxin-antitoxin system RelE/ParE family toxin -
D9K64_RS29665 34630..34857 - 228 Protein_44 transposase -
D9K64_RS27265 35029..35726 + 698 WP_181577885.1 IS1 family transposase -
D9K64_RS27270 35728..36294 + 567 WP_115272682.1 hypothetical protein -
D9K64_RS27275 36321..36527 - 207 WP_200528186.1 hypothetical protein -
D9K64_RS27280 36702..37034 - 333 WP_059065587.1 endoribonuclease MazF Toxin
D9K64_RS27285 37034..37279 - 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D9K64_RS27290 37468..37740 + 273 WP_004146533.1 hypothetical protein -
D9K64_RS27295 37721..38929 + 1209 WP_121097629.1 IS91 family transposase -
D9K64_RS27300 39049..39900 + 852 WP_121097632.1 3'-5' exonuclease -
D9K64_RS27305 40017..40280 + 264 Protein_53 hypothetical protein -
D9K64_RS29565 41300..41449 + 150 WP_168234218.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pilW 1..93621 93621
- inside IScluster/Tn - - 21172..52804 31632


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T112650 WP_059065587.1 NZ_CP032835:c37034-36702 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T112650 NZ_CP032835:c37034-36702 [Klebsiella pneumoniae]
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTGTGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGTGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT112650 WP_032454911.1 NZ_CP032835:c37279-37034 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT112650 NZ_CP032835:c37279-37034 [Klebsiella pneumoniae]
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCAGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References