Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA(toxin)
Location 30290..30876 Replicon plasmid pINF237_02
Accession NZ_CP032835
Organism Klebsiella pneumoniae strain INF237

Toxin (Protein)


Gene name hicA Uniprot ID A0A2X1Q735
Locus tag D9K64_RS27220 Protein ID WP_040174957.1
Coordinates 30631..30876 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2X1Q3U2
Locus tag D9K64_RS27215 Protein ID WP_040174959.1
Coordinates 30290..30634 (-) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K64_RS27190 26250..27470 + 1221 WP_115272108.1 ISL3 family transposase -
D9K64_RS27195 27970..28671 + 702 WP_001568041.1 DNA methylase -
D9K64_RS27200 28671..28892 + 222 WP_001568042.1 hypothetical protein -
D9K64_RS27205 28902..29321 + 420 WP_121097621.1 DUF1380 domain-containing protein -
D9K64_RS27210 29375..30142 + 768 WP_115233619.1 hypothetical protein -
D9K64_RS27215 30290..30634 - 345 WP_040174959.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
D9K64_RS27220 30631..30876 - 246 WP_040174957.1 type II toxin-antitoxin system HicA family toxin Toxin
D9K64_RS27225 31176..31436 - 261 WP_142743871.1 hypothetical protein -
D9K64_RS27230 31774..31872 - 99 Protein_40 IS1 family transposase -
D9K64_RS27235 32048..33502 + 1455 Protein_41 Tn3 family transposase -
D9K64_RS27245 33801..34064 + 264 WP_001089474.1 hypothetical protein -
D9K64_RS27250 34054..34353 + 300 WP_000119542.1 type II toxin-antitoxin system RelE/ParE family toxin -
D9K64_RS29665 34630..34857 - 228 Protein_44 transposase -
D9K64_RS27265 35029..35726 + 698 WP_181577885.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pilW 1..93621 93621
- inside IScluster/Tn - - 21172..52804 31632


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9598.36 Da        Isoelectric Point: 10.3911

>T112649 WP_040174957.1 NZ_CP032835:c30876-30631 [Klebsiella pneumoniae]
MGKNDKLLEKFINSKKTFEWTELVVLLSFLGYEKKEMQGSRVRFVHEKYKTIMLHRPHPENHIKGGALKDVKRMLKEEGL
L

Download         Length: 246 bp

>T112649 NZ_CP032835:c30876-30631 [Klebsiella pneumoniae]
ATGGGGAAAAATGATAAATTGCTGGAAAAATTTATAAACAGCAAGAAAACATTTGAATGGACAGAACTGGTTGTACTGCT
TTCATTTCTTGGTTATGAAAAAAAGGAAATGCAGGGATCACGAGTCAGATTTGTACACGAGAAATACAAAACGATAATGC
TACATCGCCCACACCCTGAAAATCATATCAAAGGCGGGGCGCTGAAAGATGTCAAGAGGATGTTAAAAGAGGAAGGTTTA
TTATGA

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 12911.64 Da        Isoelectric Point: 4.6528

>AT112649 WP_040174959.1 NZ_CP032835:c30634-30290 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFDNNILYGKLAFIGDLVTYEAETLAELEQEFKVSVDLYLQACAEDGKKPDTPFKGVFNVRVDPD
LHRRVAELALEEDLSLNAFVNKALEKEVSNHHLA

Download         Length: 345 bp

>AT112649 NZ_CP032835:c30634-30290 [Klebsiella pneumoniae]
ATGAAACATTTAAAATATAAAGGCTATTTGGGTACAGTAGAGCCAGATTTTGATAATAATATCCTGTACGGAAAACTGGC
GTTTATTGGCGATCTGGTGACTTATGAGGCTGAAACATTAGCAGAACTGGAACAGGAGTTTAAAGTATCAGTAGATTTGT
ATCTACAGGCTTGTGCAGAGGACGGGAAAAAACCAGACACCCCATTTAAAGGCGTGTTTAACGTGAGGGTAGATCCCGAT
CTGCATCGTCGTGTTGCAGAACTGGCGTTAGAAGAAGATTTATCGCTGAATGCGTTTGTTAATAAAGCGCTGGAAAAAGA
AGTCAGCAATCATCATCTGGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X1Q735

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1Q3U2

References