Detailed information of TA system TA112648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 21903..22450
• Replicon: plasmid pINF237_02
• Accession: NZ_CP032835
• Organism: Klebsiella pneumoniae strain INF237

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: D9K64_RS27160
• Protein ID: WP_115376550.1
• Coordinates: 21903..22202 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A837JUA1
• Locus tag: D9K64_RS27165
• Protein ID: WP_023317799.1
• Coordinates: 22199..22450 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K64_RS27125	17853..18485	+	633	WP_115243210.1	ParA family protein	-
D9K64_RS27130	18485..18859	+	375	WP_017901448.1	hypothetical protein	-
D9K64_RS27135	19099..20073	+	975	WP_115243209.1	StbA family protein	-
D9K64_RS27140	20077..20469	+	393	WP_115243208.1	plasmid partitioning/stability family protein	-
D9K64_RS27160	21903..22202	-	300	WP_115376550.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9K64_RS27165	22199..22450	-	252	WP_023317799.1	hypothetical protein	Antitoxin
D9K64_RS27170	22546..23817	-	1272	WP_115243206.1	Y-family DNA polymerase	-
D9K64_RS27175	23817..24242	-	426	WP_121097618.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
D9K64_RS27180	24319..25806	-	1488	WP_162985506.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pilW	1..93621	93621
	-	inside	IScluster/Tn	-	-	21172..52804	31632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11565.68 Da
• Isoelectric Point: 10.2779

>T112648 WP_115376550.1 NZ_CP032835:c22202-21903 [Klebsiella pneumoniae]
MTYTVKFREDALKEWNKLDKTIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNQLIIAIVAVGK
RERSDVYTLASERVMLPTY

Nucleotide

• Length: 300 bp

>T112648 NZ_CP032835:c22202-21903 [Klebsiella pneumoniae]
ATGACATATACCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGAACAAGCTGGATAAGACTATTCAGCAGCAGTTTGC
AAAGAAACTTAAAAAGTGCTGTGAAAACCCACACATTCCTTCAGCAAAACTACGTGGTATAAAGGATTGCTATAAAATAA
AACTGCGAGCATCTGGTTTTCGCCTCGTCTATCAGGTCATTGATAATCAACTTATAATTGCTATTGTGGCTGTAGGCAAA
CGGGAGCGAAGTGACGTTTATACACTGGCGAGTGAACGGGTAATGCTGCCAACTTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.60 Da
• Isoelectric Point: 4.2108

>AT112648 WP_023317799.1 NZ_CP032835:c22450-22199 [Klebsiella pneumoniae]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELIKLVAERSNQPLFDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT112648 NZ_CP032835:c22450-22199 [Klebsiella pneumoniae]
ATGCCACATATCATTCTAAGTGACACGACTGCCAGCGTCAGTGAATTGAAAAAAAACCCGATGGCCACAGTCAGCGCCGG
TGACGGATTCCCAGTAGCGATTCTTAACCGGAACCAGCCAGCTTTCTATTGCGTGCCTGCGGAACTCTACGAAAAGATGC
TTGATGCGCTGGATGATCAGGAACTCATTAAACTGGTTGCAGAACGCAGTAATCAACCGCTGTTTGATGTGGACCTGGAT
AAGTACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A837JUA1