Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 112017..112660 Replicon plasmid pINF237_01-VP
Accession NZ_CP032834
Organism Klebsiella pneumoniae strain INF237

Toxin (Protein)


Gene name vagD Uniprot ID A0A421H508
Locus tag D9K64_RS26875 Protein ID WP_001044773.1
Coordinates 112244..112660 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A060VSA5
Locus tag D9K64_RS26870 Protein ID WP_001261277.1
Coordinates 112017..112247 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K64_RS26845 108107..108299 + 193 Protein_102 transposase -
D9K64_RS26850 108652..109437 - 786 WP_040175061.1 site-specific integrase -
D9K64_RS26855 109483..110004 - 522 WP_121097595.1 hypothetical protein -
D9K64_RS26860 110062..110453 - 392 Protein_105 hypothetical protein -
D9K64_RS26865 110490..111434 - 945 WP_040175057.1 hypothetical protein -
D9K64_RS26870 112017..112247 + 231 WP_001261277.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D9K64_RS26875 112244..112660 + 417 WP_001044773.1 type II toxin-antitoxin system VapC family toxin Toxin
D9K64_RS26880 112719..113123 - 405 Protein_109 transposase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - clbI / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA 1..133713 133713
- flank IS/Tn - - 112677..113192 515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15227.68 Da        Isoelectric Point: 7.7805

>T112646 WP_001044773.1 NZ_CP032834:112244-112660 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPETVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T112646 NZ_CP032834:112244-112660 [Klebsiella pneumoniae]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAACCGTCCTGAAACGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8899.03 Da        Isoelectric Point: 4.5112

>AT112646 WP_001261277.1 NZ_CP032834:112017-112247 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVRELEIIREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT112646 NZ_CP032834:112017-112247 [Klebsiella pneumoniae]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
AGAGCTCGAGATCATCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGCTCATTCCTGGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421H508

Antitoxin

Source ID Structure
AlphaFold DB A0A060VSA5

References