Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 92232..93142 Replicon plasmid pINF237_01-VP
Accession NZ_CP032834
Organism Klebsiella pneumoniae strain INF237

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag D9K64_RS26720 Protein ID WP_004144067.1
Coordinates 92232..92702 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2X3DD53
Locus tag D9K64_RS26725 Protein ID WP_040175115.1
Coordinates 92699..93142 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K64_RS26675 88095..88724 - 630 Protein_76 IS256-like element IS1414 family transposase -
D9K64_RS26690 89295..89992 + 698 WP_171893191.1 IS1 family transposase -
D9K64_RS26695 90094..90492 + 399 WP_040175108.1 hypothetical protein -
D9K64_RS26705 90980..91138 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
D9K64_RS26715 91813..92121 + 309 WP_052463135.1 hypothetical protein -
D9K64_RS26720 92232..92702 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
D9K64_RS26725 92699..93142 - 444 WP_040175115.1 DUF2384 domain-containing protein Antitoxin
D9K64_RS26730 93243..93690 - 448 Protein_83 DUF4113 domain-containing protein -
D9K64_RS26745 94814..95233 + 420 Protein_85 thioredoxin family protein -
D9K64_RS29655 95223..95407 + 185 Protein_86 conjugal transfer protein -
D9K64_RS26750 95399..95983 + 585 Protein_87 thioredoxin fold domain-containing protein -
D9K64_RS26755 96000..96266 + 267 Protein_88 hypothetical protein -
D9K64_RS26765 96580..97030 + 451 Protein_89 conjugal transfer protein TraI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - clbI / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA 1..133713 133713
- inside IScluster/Tn - - 88092..94210 6118


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre

(16-147)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T112645 WP_004144067.1 NZ_CP032834:c92702-92232 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T112645 NZ_CP032834:c92702-92232 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCATGGACCGGATATGGTGCGAAAGAGGCTGGTGGACG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16466.77 Da        Isoelectric Point: 9.9958

>AT112645 WP_040175115.1 NZ_CP032834:c93142-92699 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNQALSWKVPADLMASETGAYEIIKLITRLEHGVYS

Download         Length: 444 bp

>AT112645 NZ_CP032834:c93142-92699 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTTGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCTGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCAGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGA
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A2X3DD53

References