Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | /RES-TIGR02293 |
| Location | 92232..93142 | Replicon | plasmid pINF237_01-VP |
| Accession | NZ_CP032834 | ||
| Organism | Klebsiella pneumoniae strain INF237 | ||
Toxin (Protein)
| Gene name | - | Uniprot ID | R4YBC5 |
| Locus tag | D9K64_RS26720 | Protein ID | WP_004144067.1 |
| Coordinates | 92232..92702 (-) | Length | 157 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | A0A2X3DD53 |
| Locus tag | D9K64_RS26725 | Protein ID | WP_040175115.1 |
| Coordinates | 92699..93142 (-) | Length | 148 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D9K64_RS26675 | 88095..88724 | - | 630 | Protein_76 | IS256-like element IS1414 family transposase | - |
| D9K64_RS26690 | 89295..89992 | + | 698 | WP_171893191.1 | IS1 family transposase | - |
| D9K64_RS26695 | 90094..90492 | + | 399 | WP_040175108.1 | hypothetical protein | - |
| D9K64_RS26705 | 90980..91138 | - | 159 | WP_004181898.1 | type I toxin-antitoxin system Hok family toxin | - |
| D9K64_RS26715 | 91813..92121 | + | 309 | WP_052463135.1 | hypothetical protein | - |
| D9K64_RS26720 | 92232..92702 | - | 471 | WP_004144067.1 | RES family NAD+ phosphorylase | Toxin |
| D9K64_RS26725 | 92699..93142 | - | 444 | WP_040175115.1 | DUF2384 domain-containing protein | Antitoxin |
| D9K64_RS26730 | 93243..93690 | - | 448 | Protein_83 | DUF4113 domain-containing protein | - |
| D9K64_RS26745 | 94814..95233 | + | 420 | Protein_85 | thioredoxin family protein | - |
| D9K64_RS29655 | 95223..95407 | + | 185 | Protein_86 | conjugal transfer protein | - |
| D9K64_RS26750 | 95399..95983 | + | 585 | Protein_87 | thioredoxin fold domain-containing protein | - |
| D9K64_RS26755 | 96000..96266 | + | 267 | Protein_88 | hypothetical protein | - |
| D9K64_RS26765 | 96580..97030 | + | 451 | Protein_89 | conjugal transfer protein TraI | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | clbI / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA | 1..133713 | 133713 | |
| - | inside | IScluster/Tn | - | - | 88092..94210 | 6118 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 157 a.a. Molecular weight: 17573.83 Da Isoelectric Point: 4.5152
>T112645 WP_004144067.1 NZ_CP032834:c92702-92232 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK
Download Length: 471 bp
>T112645 NZ_CP032834:c92702-92232 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCATGGACCGGATATGGTGCGAAAGAGGCTGGTGGACG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCATGGACCGGATATGGTGCGAAAGAGGCTGGTGGACG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA
Antitoxin
Download Length: 148 a.a. Molecular weight: 16466.77 Da Isoelectric Point: 9.9958
>AT112645 WP_040175115.1 NZ_CP032834:c93142-92699 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNQALSWKVPADLMASETGAYEIIKLITRLEHGVYS
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNQALSWKVPADLMASETGAYEIIKLITRLEHGVYS
Download Length: 444 bp
>AT112645 NZ_CP032834:c93142-92699 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTTGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCTGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCAGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGA
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTTGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCTGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCAGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGA
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A384IED8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2X3DD53 |