Detailed information of TA system TA112644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5123994..5124619
• Replicon: chromosome
• Accession: NZ_CP032833
• Organism: Klebsiella pneumoniae strain INF237

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2X3CDG6
• Locus tag: D9K64_RS25835
• Protein ID: WP_040172890.1
• Coordinates: 5123994..5124377 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D9K64_RS25840
• Protein ID: WP_073971928.1
• Coordinates: 5124377..5124619 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K64_RS25820	5121366..5122262	+	897	WP_040172884.1	formate dehydrogenase subunit beta	-
D9K64_RS25825	5122259..5122894	+	636	WP_040172888.1	formate dehydrogenase cytochrome b556 subunit	-
D9K64_RS25830	5122891..5123820	+	930	WP_121097509.1	formate dehydrogenase accessory protein FdhE	-
D9K64_RS25835	5123994..5124377	-	384	WP_040172890.1	type II toxin-antitoxin system VapC family toxin	Toxin
D9K64_RS25840	5124377..5124619	-	243	WP_073971928.1	ribbon-helix-helix domain-containing protein	Antitoxin
D9K64_RS25850	5124823..5125740	+	918	WP_040172896.1	alpha/beta hydrolase	-
D9K64_RS25855	5125755..5126696	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
D9K64_RS25860	5126741..5127178	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D9K64_RS25865	5127175..5128035	-	861	WP_004146232.1	virulence factor BrkB family protein	-
D9K64_RS25870	5128029..5128628	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14420.64 Da
• Isoelectric Point: 8.5338

>T112644 WP_040172890.1 NZ_CP032833:c5124377-5123994 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQRHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T112644 NZ_CP032833:c5124377-5123994 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACGCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9553.80 Da
• Isoelectric Point: 4.3509

>AT112644 WP_073971928.1 NZ_CP032833:c5124619-5124377 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNVPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT112644 NZ_CP032833:c5124619-5124377 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACGTTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A2X3CDG6