Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3548110..3548729 Replicon chromosome
Accession NZ_CP032833
Organism Klebsiella pneumoniae strain INF237

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag D9K64_RS17920 Protein ID WP_002892050.1
Coordinates 3548511..3548729 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag D9K64_RS17915 Protein ID WP_002892066.1
Coordinates 3548110..3548484 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K64_RS17905 3543701..3546847 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
D9K64_RS17910 3547017..3547714 + 698 WP_171893191.1 IS1 family transposase -
D9K64_RS17915 3548110..3548484 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
D9K64_RS17920 3548511..3548729 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
D9K64_RS17925 3548888..3549454 + 567 WP_002892030.1 maltose O-acetyltransferase -
D9K64_RS29245 3549426..3549566 - 141 WP_032409038.1 hypothetical protein -
D9K64_RS17930 3549587..3550057 + 471 WP_002892026.1 YlaC family protein -
D9K64_RS17935 3550032..3551483 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
D9K64_RS17940 3551584..3552282 + 699 WP_115233840.1 GNAT family N-acetyltransferase -
D9K64_RS17945 3552279..3552419 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
D9K64_RS17950 3552419..3552682 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3547337..3547714 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T112641 WP_002892050.1 NZ_CP032833:3548511-3548729 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T112641 NZ_CP032833:3548511-3548729 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT112641 WP_002892066.1 NZ_CP032833:3548110-3548484 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT112641 NZ_CP032833:3548110-3548484 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References