Detailed information of TA system TA112622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 127699..128342
• Replicon: plasmid pINF078-VP
• Accession: NZ_CP032832
• Organism: Klebsiella pneumoniae strain INF078

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: D9K63_RS26945
• Protein ID: WP_086624931.1
• Coordinates: 127926..128342 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: D9K63_RS26940
• Protein ID: WP_086624932.1
• Coordinates: 127699..127929 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K63_RS26920	122753..124948	-	2196	WP_121016050.1	aldehyde dehydrogenase family protein	-
D9K63_RS26925	124945..126423	-	1479	WP_086624935.1	cytosine permease	-
D9K63_RS26930	126462..126845	-	384	WP_086624934.1	nuclear transport factor 2 family protein	-
D9K63_RS26940	127699..127929	+	231	WP_086624932.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D9K63_RS26945	127926..128342	+	417	WP_086624931.1	type II toxin-antitoxin system VapC family toxin	Toxin
D9K63_RS26950	128515..128811	-	297	WP_086624930.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D9K63_RS26955	128798..129064	-	267	WP_086624929.1	type II toxin-antitoxin system ParD family antitoxin	-
D9K63_RS26960	129359..129619	+	261	WP_117089439.1	hypothetical protein	-
D9K63_RS29735	129616..130373	+	758	Protein_125	hypothetical protein	-
D9K63_RS26975	130507..130674	+	168	Protein_126	DNA-binding protein	-
D9K63_RS26980	130670..131089	-	420	WP_117089440.1	NUDIX domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroB	1..399913	399913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15008.54 Da
• Isoelectric Point: 9.2957

>T112622 WP_086624931.1 NZ_CP032832:127926-128342 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGAKASPRHIQLVDAFCARLDAILPWDRA
AVDVTTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T112622 NZ_CP032832:127926-128342 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCG
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGTCACTACGGACATACGCGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8707.72 Da
• Isoelectric Point: 4.3533

>AT112622 WP_086624932.1 NZ_CP032832:127699-127929 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPSWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT112622 NZ_CP032832:127699-127929 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGTCCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA