Detailed information of TA system TA112618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 59183..59826
• Replicon: plasmid pINF078-VP
• Accession: NZ_CP032832
• Organism: Klebsiella pneumoniae strain INF078

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: D9K63_RS26605
• Protein ID: WP_086624931.1
• Coordinates: 59410..59826 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: D9K63_RS26600
• Protein ID: WP_086624932.1
• Coordinates: 59183..59413 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K63_RS26580	54960..56432	-	1473	WP_086624936.1	aldehyde dehydrogenase family protein	-
D9K63_RS26585	56429..57907	-	1479	WP_086624935.1	cytosine permease	-
D9K63_RS26590	57946..58329	-	384	WP_086624934.1	nuclear transport factor 2 family protein	-
D9K63_RS26600	59183..59413	+	231	WP_086624932.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D9K63_RS26605	59410..59826	+	417	WP_086624931.1	type II toxin-antitoxin system VapC family toxin	Toxin
D9K63_RS26610	59999..60295	-	297	WP_086624930.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D9K63_RS26615	60282..60548	-	267	WP_086624929.1	type II toxin-antitoxin system ParD family antitoxin	-
D9K63_RS26620	60843..61103	+	261	WP_117089439.1	hypothetical protein	-
D9K63_RS29715	61100..61857	+	758	Protein_65	hypothetical protein	-
D9K63_RS26635	61991..62158	+	168	Protein_66	DNA-binding protein	-
D9K63_RS26640	62154..62573	-	420	WP_117089440.1	NUDIX domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroN / iroE / iroD / iroC / iroB	1..399913	399913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15008.54 Da
• Isoelectric Point: 9.2957

>T112618 WP_086624931.1 NZ_CP032832:59410-59826 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGAKASPRHIQLVDAFCARLDAILPWDRA
AVDVTTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T112618 NZ_CP032832:59410-59826 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCG
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGTCACTACGGACATACGCGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8707.72 Da
• Isoelectric Point: 4.3533

>AT112618 WP_086624932.1 NZ_CP032832:59183-59413 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPSWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT112618 NZ_CP032832:59183-59413 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGTCCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA