Detailed information of TA system TA112617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5217423..5218048
• Replicon: chromosome
• Accession: NZ_CP032831
• Organism: Klebsiella pneumoniae strain INF078

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: D9K63_RS25870
• Protein ID: WP_002882817.1
• Coordinates: 5217423..5217806 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D9K63_RS25875
• Protein ID: WP_162557435.1
• Coordinates: 5217806..5218048 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K63_RS25855	5214789..5215691	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
D9K63_RS25860	5215688..5216323	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
D9K63_RS25865	5216320..5217249	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
D9K63_RS25870	5217423..5217806	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
D9K63_RS25875	5217806..5218048	-	243	WP_162557435.1	ribbon-helix-helix domain-containing protein	Antitoxin
D9K63_RS25880	5218253..5219170	+	918	WP_032417884.1	alpha/beta hydrolase	-
D9K63_RS25885	5219185..5220126	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
D9K63_RS25890	5220171..5220608	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D9K63_RS25895	5220605..5221465	-	861	WP_004146232.1	virulence factor BrkB family protein	-
D9K63_RS25900	5221459..5222058	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T112617 WP_002882817.1 NZ_CP032831:c5217806-5217423 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T112617 NZ_CP032831:c5217806-5217423 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9557.79 Da
• Isoelectric Point: 4.3509

>AT112617 WP_162557435.1 NZ_CP032831:c5218048-5217806 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNISRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT112617 NZ_CP032831:c5218048-5217806 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTTCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8