Detailed information of TA system TA112614

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4654683..4655386
• Replicon: chromosome
• Accession: NZ_CP032831
• Organism: Klebsiella pneumoniae strain INF078

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: D9K63_RS23110
• Protein ID: WP_048261918.1
• Coordinates: 4654683..4655024 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: D9K63_RS23115
• Protein ID: WP_063308407.1
• Coordinates: 4655045..4655386 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K63_RS23075	4649883..4650557	+	675	WP_004186809.1	hypothetical protein	-
D9K63_RS23080	4650559..4650906	+	348	WP_004186808.1	hypothetical protein	-
D9K63_RS23085	4650909..4651388	+	480	WP_004192264.1	type VI secretion system tube protein Hcp	-
D9K63_RS23095	4651938..4653049	-	1112	Protein_4446	IS3 family transposase	-
D9K63_RS23105	4653417..4654424	-	1008	WP_038422858.1	restriction endonuclease	-
D9K63_RS23110	4654683..4655024	-	342	WP_048261918.1	TA system toxin CbtA family protein	Toxin
D9K63_RS23115	4655045..4655386	-	342	WP_063308407.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D9K63_RS23120	4655397..4655939	-	543	WP_117247367.1	DNA repair protein RadC	-
D9K63_RS23125	4655952..4656392	-	441	WP_117247366.1	antirestriction protein	-
D9K63_RS23130	4656424..4657245	-	822	WP_117247365.1	DUF945 domain-containing protein	-
D9K63_RS23135	4657346..4657576	-	231	WP_117247364.1	DUF905 domain-containing protein	-
D9K63_RS23140	4657648..4658097	-	450	WP_117247363.1	hypothetical protein	-
D9K63_RS23145	4658094..4658546	-	453	WP_117247362.1	hypothetical protein	-
D9K63_RS23150	4658583..4659152	-	570	WP_117247361.1	hypothetical protein	-
D9K63_RS23155	4659152..4659856	-	705	WP_014226755.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4650559..4686285	35726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12750.69 Da
• Isoelectric Point: 8.0324

>T112614 WP_048261918.1 NZ_CP032831:c4655024-4654683 [Klebsiella pneumoniae]
MKTLPATTPQAATLCLSPVTVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWEEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T112614 NZ_CP032831:c4655024-4654683 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTAACTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAGCATTATGGCCTGACACTCAACGACACGCCGTTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCTGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGAGGATTC
AACTGGGAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12722.34 Da
• Isoelectric Point: 6.4739

>AT112614 WP_063308407.1 NZ_CP032831:c4655386-4655045 [Klebsiella pneumoniae]
MKSTDSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT112614 NZ_CP032831:c4655386-4655045 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCCAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGAAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCGCATTTTATCAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA