Detailed information of TA system TA112600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 703499..704015
• Replicon: plasmid unnamed1
• Accession: NZ_CP032828
• Organism: Sphingomonas sp. YZ-8

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: D3Y57_RS04645
• Protein ID: WP_121151780.1
• Coordinates: 703499..703750 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: D3Y57_RS04650
• Protein ID: WP_121151782.1
• Coordinates: 703740..704015 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3Y57_RS04615	698893..699429	-	537	WP_121151772.1	TetR/AcrR family transcriptional regulator	-
D3Y57_RS04620	699533..699708	+	176	Protein_678	site-specific integrase	-
D3Y57_RS04630	700142..701443	+	1302	WP_121151776.1	integrase arm-type DNA-binding domain-containing protein	-
D3Y57_RS04635	701547..702158	-	612	WP_162986960.1	hypothetical protein	-
D3Y57_RS04640	702473..703278	-	806	Protein_681	DNA adenine methylase	-
D3Y57_RS04645	703499..703750	+	252	WP_121151780.1	BrnT family toxin	Toxin
D3Y57_RS04650	703740..704015	+	276	WP_121151782.1	BrnA antitoxin family protein	Antitoxin
D3Y57_RS04655	704303..704482	+	180	WP_162986961.1	hypothetical protein	-
D3Y57_RS04660	704489..704815	+	327	WP_121151786.1	hypothetical protein	-
D3Y57_RS20030	704812..704973	+	162	WP_162986962.1	hypothetical protein	-
D3Y57_RS04665	704970..705611	-	642	WP_121151788.1	SOS response-associated peptidase	-
D3Y57_RS04670	705685..706701	-	1017	WP_121151790.1	DUF2190 family protein	-
D3Y57_RS04675	706737..707042	-	306	WP_121151792.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..849778	849778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (14-90)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9347.80 Da
• Isoelectric Point: 10.2981

>T112600 WP_121151780.1 NZ_CP032828:703499-703750 [Sphingomonas sp. YZ-8]
MDIEFDPAKDAANIAKHGVSLDRAVELAGIVVVEDTRFAERRLRLYGTIDGIAHCAAVTMRGNVVRVINLRRAHRKEVRR
YGF

Nucleotide

• Length: 252 bp

>T112600 NZ_CP032828:703499-703750 [Sphingomonas sp. YZ-8]
ATGGACATCGAGTTTGACCCCGCGAAGGACGCAGCAAATATCGCCAAGCATGGCGTGTCGCTGGATCGGGCAGTCGAACT
CGCGGGGATCGTGGTCGTAGAAGACACCCGGTTCGCTGAACGTCGCCTTCGTCTCTACGGGACCATCGACGGAATTGCCC
ATTGTGCGGCGGTGACGATGCGCGGCAACGTCGTCCGCGTCATCAATCTACGCCGCGCTCATCGCAAGGAGGTACGTCGC
TATGGTTTCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9942.08 Da
• Isoelectric Point: 8.8077

>AT112600 WP_121151782.1 NZ_CP032828:703740-704015 [Sphingomonas sp. YZ-8]
MVSDKHGPVVFDSDNPEWTAEDFAKARPIADFPELSAAFPNGSKPRGRPKGSVSSSKTLVSLRLDNDVLERFRATGPGWQ
SRINEALRSAT

Nucleotide

• Length: 276 bp

>AT112600 NZ_CP032828:703740-704015 [Sphingomonas sp. YZ-8]
ATGGTTTCTGACAAACATGGTCCTGTCGTTTTCGACAGTGACAACCCCGAATGGACCGCTGAAGATTTCGCGAAGGCCCG
CCCAATTGCGGACTTTCCGGAGCTGTCGGCTGCATTTCCGAATGGCAGTAAGCCCCGTGGTCGACCAAAAGGCTCTGTCA
GCTCAAGCAAGACGCTGGTGTCGCTGCGGTTGGATAACGATGTCCTGGAGCGTTTCAGGGCAACGGGGCCTGGATGGCAG
TCGCGGATCAATGAGGCGCTGCGGTCTGCCACTTAG