Detailed information of TA system TA112591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1976230..1976759
• Replicon: chromosome
• Accession: NZ_CP032821
• Organism: Staphylococcus aureus strain JDFM SA01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D5S13_RS09725
• Protein ID: WP_000621175.1
• Coordinates: 1976397..1976759 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: D5S13_RS09720
• Protein ID: WP_000948331.1
• Coordinates: 1976230..1976400 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5S13_RS09690 (D5S13_01855)	1971266..1971826	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
D5S13_RS09695 (D5S13_01856)	1972035..1972514	+	480	WP_001287079.1	hypothetical protein	-
D5S13_RS09700 (D5S13_01857)	1972507..1974090	+	1584	WP_001294620.1	PH domain-containing protein	-
D5S13_RS09705 (D5S13_01858)	1974077..1974568	+	492	WP_001205912.1	PH domain-containing protein	-
D5S13_RS09710 (D5S13_01859)	1974572..1974931	+	360	WP_000581197.1	holo-ACP synthase	-
D5S13_RS09715 (D5S13_01860)	1974997..1976145	+	1149	WP_263258444.1	alanine racemase	-
D5S13_RS09720 (D5S13_01861)	1976230..1976400	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
D5S13_RS09725 (D5S13_01862)	1976397..1976759	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D5S13_RS09730 (D5S13_01863)	1977109..1978110	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
D5S13_RS09735 (D5S13_01864)	1978229..1978555	+	327	WP_001052491.1	anti-sigma factor antagonist	-
D5S13_RS09740 (D5S13_01865)	1978557..1979036	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
D5S13_RS09745 (D5S13_01866)	1979011..1979781	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T112591 WP_000621175.1 NZ_CP032821:1976397-1976759 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T112591 NZ_CP032821:1976397-1976759 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT112591 WP_000948331.1 NZ_CP032821:1976230-1976400 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT112591 NZ_CP032821:1976230-1976400 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1