Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1956487..1957001 Replicon chromosome
Accession NZ_CP032821
Organism Staphylococcus aureus strain JDFM SA01

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag D5S13_RS09635 Protein ID WP_000581792.1
Coordinates 1956792..1957001 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag D5S13_RS09630 Protein ID WP_000138932.1
Coordinates 1956487..1956780 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5S13_RS09610 (D5S13_01839) 1951852..1952493 + 642 WP_000483156.1 thiamine phosphate synthase -
D5S13_RS09615 (D5S13_01840) 1952579..1953451 + 873 WP_000725800.1 membrane protein insertase YidC -
D5S13_RS09620 (D5S13_01841) 1954163..1954810 - 648 WP_001187630.1 HD domain-containing protein -
D5S13_RS09625 1954838..1956321 - 1484 Protein_1888 cardiolipin synthase -
D5S13_RS09630 (D5S13_01844) 1956487..1956780 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
D5S13_RS09635 (D5S13_01845) 1956792..1957001 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
D5S13_RS09640 (D5S13_01846) 1957129..1957266 + 138 WP_000828354.1 Lmo0850 family protein -
D5S13_RS09645 (D5S13_01847) 1957530..1958732 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
D5S13_RS09650 (D5S13_01848) 1959050..1960120 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
D5S13_RS09655 (D5S13_01849) 1960135..1961493 + 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T112590 WP_000581792.1 NZ_CP032821:1956792-1957001 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T112590 NZ_CP032821:1956792-1957001 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT112590 WP_000138932.1 NZ_CP032821:1956487-1956780 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT112590 NZ_CP032821:1956487-1956780 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References