Detailed information of TA system TA112580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 2330..2877
• Replicon: plasmid pERL03-1416-2
• Accession: NZ_CP032813
• Organism: Escherichia coli strain ERL03-1416

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: D8Z67_RS27915
• Protein ID: WP_000970003.1
• Coordinates: 2596..2877 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1X3K043
• Locus tag: D8Z67_RS27910
• Protein ID: WP_061360246.1
• Coordinates: 2330..2599 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z67_RS27885	1..261	+	261	WP_000083819.1	replication regulatory protein RepA	-
D8Z67_RS27895	490..564	+	75	WP_032144212.1	RepA leader peptide Tap	-
D8Z67_RS27900	557..1414	+	858	WP_151567926.1	incFII family plasmid replication initiator RepA	-
D8Z67_RS28560	2117..2275	+	159	WP_072664725.1	hypothetical protein	-
D8Z67_RS27910	2330..2599	+	270	WP_061360246.1	hypothetical protein	Antitoxin
D8Z67_RS27915	2596..2877	+	282	WP_000970003.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D8Z67_RS27920	3141..4328	+	1188	WP_000905831.1	hypothetical protein	-
D8Z67_RS27925	4418..5329	+	912	WP_024244186.1	toll/interleukin-1 receptor domain-containing protein	-
D8Z67_RS27930	5471..5866	-	396	WP_000445930.1	plasmid partitioning/stability family protein	-
D8Z67_RS27935	5866..6825	-	960	WP_000921958.1	plasmid segregation protein ParM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..62779	62779

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10863.48 Da
• Isoelectric Point: 8.4615

>T112580 WP_000970003.1 NZ_CP032813:2596-2877 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLTAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T112580 NZ_CP032813:2596-2877 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAC
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10284.64 Da
• Isoelectric Point: 7.2057

>AT112580 WP_061360246.1 NZ_CP032813:2330-2599 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDMLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT112580 NZ_CP032813:2330-2599 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCAGAACTGAAGGATGCTTTTATGGCCGCAGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATATGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1X3K043