Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2628214..2628685 Replicon chromosome
Accession NZ_CP032811
Organism Escherichia coli strain ERL03-1416

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag D8Z67_RS13535 Protein ID WP_001303511.1
Coordinates 2628214..2628492 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag D8Z67_RS13540 Protein ID WP_001302048.1
Coordinates 2628494..2628685 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z67_RS13505 2625150..2626235 - 1086 Protein_2592 exonuclease -
D8Z67_RS13510 2626328..2626519 - 192 WP_001090200.1 DUF1482 family protein -
D8Z67_RS13515 2626516..2626704 - 189 WP_000449192.1 cell division inhibition protein DicB -
D8Z67_RS13525 2627273..2627491 - 219 WP_001171930.1 hypothetical protein -
D8Z67_RS13530 2627563..2627862 - 300 WP_001240334.1 hypothetical protein -
D8Z67_RS13535 2628214..2628492 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8Z67_RS13540 2628494..2628685 - 192 WP_001302048.1 hypothetical protein Antitoxin
D8Z67_RS13545 2628706..2629077 - 372 WP_001169686.1 hypothetical protein -
D8Z67_RS13550 2629175..2629477 + 303 WP_000172738.1 transcriptional regulator -
D8Z67_RS13555 2629474..2629899 + 426 WP_000693943.1 Rha family transcriptional regulator -
D8Z67_RS13560 2629922..2630884 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
D8Z67_RS13565 2630891..2631631 + 741 WP_000788938.1 ATP-binding protein -
D8Z67_RS13570 2631657..2632426 + 770 Protein_2604 DUF1627 domain-containing protein -
D8Z67_RS13575 2632442..2632837 + 396 WP_001118159.1 DUF977 family protein -
D8Z67_RS13580 2632894..2633478 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2612022..2678381 66359


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T112565 WP_001303511.1 NZ_CP032811:c2628492-2628214 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T112565 NZ_CP032811:c2628492-2628214 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT112565 WP_001302048.1 NZ_CP032811:c2628685-2628494 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT112565 NZ_CP032811:c2628685-2628494 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References