Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2447501..2447972 Replicon chromosome
Accession NZ_CP032805
Organism Escherichia coli strain ERL05-0623

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag D8Z69_RS12335 Protein ID WP_001303511.1
Coordinates 2447501..2447779 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag D8Z69_RS12340 Protein ID WP_001302048.1
Coordinates 2447781..2447972 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z69_RS12300 2442726..2442977 - 252 WP_000005552.1 excisionase family protein -
D8Z69_RS12305 2443050..2445521 - 2472 WP_000048458.1 exonuclease -
D8Z69_RS12310 2445614..2445805 - 192 WP_001090200.1 DUF1482 family protein -
D8Z69_RS12315 2445802..2445990 - 189 WP_000449192.1 cell division inhibition protein DicB -
D8Z69_RS12325 2446559..2446777 - 219 WP_001171930.1 hypothetical protein -
D8Z69_RS12330 2446849..2447148 - 300 WP_001240334.1 hypothetical protein -
D8Z69_RS12335 2447501..2447779 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8Z69_RS12340 2447781..2447972 - 192 WP_001302048.1 hypothetical protein Antitoxin
D8Z69_RS12345 2447993..2448364 - 372 WP_001169686.1 hypothetical protein -
D8Z69_RS12350 2448462..2448764 + 303 WP_000172738.1 transcriptional regulator -
D8Z69_RS12355 2448761..2449186 + 426 WP_000693943.1 Rha family transcriptional regulator -
D8Z69_RS12360 2449209..2450171 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
D8Z69_RS12365 2450178..2450918 + 741 WP_000788938.1 ATP-binding protein -
D8Z69_RS12370 2450944..2451713 + 770 Protein_2375 DUF1627 domain-containing protein -
D8Z69_RS12375 2451729..2452124 + 396 WP_001118159.1 DUF977 family protein -
D8Z69_RS12380 2452181..2452765 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2432621..2489028 56407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T112487 WP_001303511.1 NZ_CP032805:c2447779-2447501 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T112487 NZ_CP032805:c2447779-2447501 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT112487 WP_001302048.1 NZ_CP032805:c2447972-2447781 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT112487 NZ_CP032805:c2447972-2447781 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References