Detailed information of TA system TA112450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2614758..2615229
• Replicon: chromosome
• Accession: NZ_CP032803
• Organism: Escherichia coli strain ERL05-1306

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: D8Z70_RS13515
• Protein ID: WP_001303511.1
• Coordinates: 2614758..2615036 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: D8Z70_RS13520
• Protein ID: WP_001302048.1
• Coordinates: 2615038..2615229 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z70_RS13475	2609764..2609948	-	185	Protein_2573	DUF3596 domain-containing protein	-
D8Z70_RS13480	2609983..2610234	-	252	WP_000005552.1	excisionase family protein	-
D8Z70_RS13485	2610307..2612778	-	2472	WP_000048458.1	exonuclease	-
D8Z70_RS13490	2612871..2613062	-	192	WP_001090200.1	DUF1482 family protein	-
D8Z70_RS13495	2613059..2613247	-	189	WP_000449192.1	cell division inhibition protein DicB	-
D8Z70_RS13505	2613816..2614034	-	219	WP_001171930.1	hypothetical protein	-
D8Z70_RS13510	2614106..2614405	-	300	WP_001240334.1	hypothetical protein	-
D8Z70_RS13515	2614758..2615036	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D8Z70_RS13520	2615038..2615229	-	192	WP_001302048.1	hypothetical protein	Antitoxin
D8Z70_RS13525	2615250..2615621	-	372	WP_001169686.1	hypothetical protein	-
D8Z70_RS13530	2615719..2616021	+	303	WP_000172738.1	transcriptional regulator	-
D8Z70_RS13535	2616018..2616443	+	426	WP_000693943.1	Rha family transcriptional regulator	-
D8Z70_RS13540	2616466..2617428	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
D8Z70_RS13545	2617435..2618175	+	741	WP_000788938.1	ATP-binding protein	-
D8Z70_RS13550	2618201..2618970	+	770	Protein_2587	DUF1627 domain-containing protein	-
D8Z70_RS13555	2618986..2619381	+	396	WP_001118159.1	DUF977 family protein	-
D8Z70_RS13560	2619438..2620022	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2599890..2736790	136900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T112450 WP_001303511.1 NZ_CP032803:c2615036-2614758 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T112450 NZ_CP032803:c2615036-2614758 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT112450 WP_001302048.1 NZ_CP032803:c2615229-2615038 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT112450 NZ_CP032803:c2615229-2615038 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0