Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51325..51850 Replicon plasmid pERL06-2442
Accession NZ_CP032802
Organism Escherichia coli strain ERL06-2442

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag D8Z71_RS28355 Protein ID WP_001159868.1
Coordinates 51545..51850 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag D8Z71_RS28350 Protein ID WP_000813634.1
Coordinates 51325..51543 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z71_RS28315 46608..46838 + 231 WP_010891288.1 hypothetical protein -
D8Z71_RS28320 46959..47699 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
D8Z71_RS28325 47984..48961 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
D8Z71_RS28335 49369..49569 - 201 WP_000708307.1 hypothetical protein -
D8Z71_RS28340 49566..50186 - 621 WP_001248529.1 hypothetical protein -
D8Z71_RS28345 50183..50866 - 684 WP_010891291.1 DNA-binding protein -
D8Z71_RS28350 51325..51543 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
D8Z71_RS28355 51545..51850 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
D8Z71_RS28360 51851..52657 + 807 WP_000016989.1 site-specific integrase -
D8Z71_RS28365 52773..53135 + 363 Protein_52 RepB family plasmid replication initiator protein -
D8Z71_RS28370 53723..54889 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
D8Z71_RS28375 54889..55860 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..90995 90995


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T112432 WP_001159868.1 NZ_CP032802:51545-51850 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T112432 NZ_CP032802:51545-51850 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT112432 WP_000813634.1 NZ_CP032802:51325-51543 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT112432 NZ_CP032802:51325-51543 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References